Latanoprost rescues retinal neuro-glial cells from apoptosis by inhibiting caspase-3, which is mediated by p44/p42 mitogen-activated protein kinase

The purpose of this study was to investigate whether latanoprost, a prostaglandin F2α analogue, has a direct anti-apoptotic effect both in retinal neuro-glial cells in culture and in diabetic retina. R28 cells, immortalized retinal neuroglial progenitor cells, were induced apoptosis by 24h serum dep...

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Veröffentlicht in:	Experimental eye research 2006-11, Vol.83 (5), p.1108-1117
Hauptverfasser:	Nakanishi, Yoriko, Nakamura, Makoto, Mukuno, Hirokazu, Kanamori, Akiyasu, Seigel, Gail M., Negi, Akira
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals apoptosis Apoptosis - drug effects Caspase Inhibitors Cell Line Diabetes Mellitus, Experimental - physiopathology diabetic retinopathy Diabetic Retinopathy - physiopathology Dose-Response Relationship, Drug Enzyme Inhibitors - pharmacology Extracellular Signal-Regulated MAP Kinases - metabolism Immunohistochemistry - methods latanoprost Male mitogen-activated protein kinase Mitogen-Activated Protein Kinase 1 - metabolism Mitogen-Activated Protein Kinase 3 - metabolism Neuroglia - drug effects Neuroglia - physiology Neuroprotective Agents - pharmacology Ophthalmic Solutions - pharmacology Prostaglandins F, Synthetic - pharmacology Rats Rats, Sprague-Dawley Retina - cytology Retina - drug effects Retinal Ganglion Cells - drug effects Retinal Ganglion Cells - physiology retinal neurodegeneration Stem Cells - drug effects Stem Cells - physiology
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Zusammenfassung:	The purpose of this study was to investigate whether latanoprost, a prostaglandin F2α analogue, has a direct anti-apoptotic effect both in retinal neuro-glial cells in culture and in diabetic retina. R28 cells, immortalized retinal neuroglial progenitor cells, were induced apoptosis by 24h serum deprivation. Serum withdrawal made up to 15% of R28 cells pyknotic and activated caspase-3 immunoreactive, and latanoprost acid suppressed apoptosis with dose dependency at an optimum concentration of 1.0μM (P
ISSN:	0014-4835 1096-0007
DOI:	10.1016/j.exer.2006.05.018