HER-2 DNA quantification of paraffin-embedded breast carcinomas with LightCycler real-time PCR in comparison to immunohistochemistry and chromogenic in situ hybridization
To compare the detection of HER-2 status by real-time PCR, on paraffin-embedded breast carcinomas, in respect to immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH). Paraffin-embedded breast carcinomas collected from 85 patients diagnosed with early stage breast cancer were analy...
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Veröffentlicht in: | Clinical biochemistry 2006-09, Vol.39 (9), p.942-946 |
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Sprache: | eng |
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Zusammenfassung: | To compare the detection of
HER-2 status by real-time PCR, on paraffin-embedded breast carcinomas, in respect to immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH).
Paraffin-embedded breast carcinomas collected from 85 patients diagnosed with early stage breast cancer were analyzed for
HER-2 gene amplification by real-time PCR and CISH, as well as for
HER-2 protein expression by IHC.
HER-2 gene amplification was observed in 19 (22.4%) of 85 breast cancer patients by real-time PCR and in 19 (22.4%) of 85 patients by CISH. Strong (3+)
HER-2 protein over-expression was observed in 13 (15.3%) out of 85 patients. Moreover, there were 4 out of 85 (4.7%) patients that had moderate (2+)
HER-2 protein over-expression, while 68 out of 85 (80%) patients had no
HER-2 protein over-expression by IHC. There were strong concordance rates between real-time PCR and IHC (79/85, 92.9%,
p
<
0.0001) and real-time PCR and CISH (77/85, 90.6%,
p
<
0.0001). The concordance rate between the three methods was 90.6% (
p
<
0.0001).
Our data show that the results obtained for amplification of
HER-2 by real-time PCR on the LightCycler are comparable to those obtained by IHC and CISH. |
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ISSN: | 0009-9120 1873-2933 |
DOI: | 10.1016/j.clinbiochem.2006.06.001 |