Quantum Dots Monitor TrkA Receptor Dynamics in the Interior of Neural PC12 Cells

Can quantum dots (QDs) serve as physiologically relevant receptor probes in the interior of cells? We directly visualize endocytosis, redistribution, and shuttling of QD bound-TrkA receptors to PC12 neural processes and far-reaching growth cone tips. Internalized QDs are contained in microtubule-ass...

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Veröffentlicht in:	Nano letters 2006-09, Vol.6 (9), p.2049-2059
Hauptverfasser:	Sundara Rajan, Sujata, Vu, Tania Q
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cross-disciplinary physics: materials science rheology Exact sciences and technology Immunoassay - methods Kinetics Materials science Metabolic Clearance Rate Microscopy, Fluorescence - methods Molecular Probe Techniques Nanoscale materials and structures: fabrication and characterization Neurons - metabolism PC12 Cells Physics Quantum Dots Rats Receptor, trkA - metabolism
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Beschreibung
Zusammenfassung:	Can quantum dots (QDs) serve as physiologically relevant receptor probes in the interior of cells? We directly visualize endocytosis, redistribution, and shuttling of QD bound-TrkA receptors to PC12 neural processes and far-reaching growth cone tips. Internalized QDs are contained in microtubule-associated vesicles and possess transport properties that reflect TrkA receptor dynamics. This opens up new possibilities for the development of QD platforms as molecular tools to image biochemical signaling and transport cargo in the cell interior.
ISSN:	1530-6984 1530-6992
DOI:	10.1021/nl0612650