Cryptococcus mujuensis sp. nov. and Cryptococcus cuniculi sp. nov., basidiomycetous yeasts isolated from wild rabbit faeces

1 Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Yusong, Daejon 305-333, Republic of Korea 2 Proteome Research Laboratory, Korea Research Institute of Bioscience and Biotechnology, Yusong, Daejon 305-333, Republic of Korea 3 The School of Bioresource Science, Andong National University, Andong 760-749, Republic of Korea 4 Department of Anatomy, School of Medicine, Chungnam National University, Daejon 301-131, Republic of Korea Correspondence Kee-Sun Shin ksshin{at}kribb.re.kr Two previously undescribed anamorphic yeasts, strains T-11 T and T-26 T , recovered from wild rabbit faecal pellets collected in Muju, Korea, were identified using phenotypic and molecular taxonomic methods. The isolates were characterized by the proliferation of budding cells, positive diazonium blue B and urease reactions, the presence of Q-10 as the major ubiquinone, the presence of xylose in whole-cell hydrolysates and the inability to ferment sugars. Phylogenetic analyses based on 26S rRNA gene partial sequences revealed that strain T-11 T was located in the Bulleromyces clade and was related to Sirobasidium intermedium , Tremella exigua , Cryptococcus cellulolyticus and Bullera pseudoalba . Strain T-26 T was located in the Mesenterica clade and was closely related to Cryptococcus sp. F6 and Cryptococcus heveanensis CBS 8976. Sequence divergence values of more than 4 % from other described Cryptococcus species, together with the phenotypic differences, showed that the isolated yeasts represent previously unrecognized members of this genus. Therefore, two novel yeast species are proposed: Cryptococcus mujuensis sp. nov., with strain T-11 T (=KCTC 17231 T =CBS 10308 T ) as the type strain, and Cryptococcus cuniculi sp. nov., with strain T-26 T (=KCTC 17232 T =CBS 10309 T ) as the type strain. The GenBank/EMBL/DDBJ accession numbers for the 26S rRNA gene D1/D2 domain sequences of strains T-11 T and T-26 T are DQ333884 and DQ333885 , respectively.