Burkholderia oklahomensis sp. nov., a Burkholderia pseudomallei-like species formerly known as the Oklahoma strain of Pseudomonas pseudomallei

Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC), 1600 Clifton Rd, NE, MS-D11, Atlanta, GA 30333, USA Correspondence Jay E. Gee JGee1{at}cdc.gov C6786, the clinical isolate of the ‘Oklahoma’ strain of Pseudomonas (now Burkholderia ) pseudomallei , was originally isolated in 1973 from a wound infection resulting from a farming accident in Oklahoma, USA. Environmental isolates C7532 and C7533 from the Oklahoma accident site were found to match C6786. These three isolates and a clinical isolate originally identified as B. pseudomallei that was recovered from a person in Georgia, USA, involved in an automobile accident were characterized by biochemical, 16S rRNA gene sequencing, multilocus sequence typing and DNA–DNA hybridization analyses. Results indicated that these strains comprise a novel species. The name Burkholderia oklahomensis sp. nov. is proposed, with strain C6786 T (=LMG 23618 T =NCTC 13387 T =CCUG 51349 T ) as the type strain. Abbreviations: MLST, multilocus sequence typing; RBR, relative binding ratio; ST, sequence type The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains C6786 T , C7532, C7533, E0147 and ATCC 23343 T are DQ108388 , DQ108389 , DQ108390 , DQ108391 and DQ108392 , respectively.