Cell Mechanics Using Atomic Force Microscopy-Based Single-Cell Compression

Cell Mechanics Using Atomic Force Microscopy-Based Single-Cell Compression

We report herein the establishment of a single-cell compression method based on force measurements in atomic force microscopy (AFM). The high-resolution bright-field or confocal laser scanning microscopy guides the location of the AFM probe and then monitors the deformation of cell shape, while micr...

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Veröffentlicht in:Langmuir 2006-09, Vol.22 (19), p.8151-8155
Hauptverfasser: Lulevich, Valentin, Zink, Tiffany, Chen, Huan-Yuan, Liu, Fu-Tong, Liu, Gang-yu
Format: Artikel
Sprache:eng
Schlagworte:
Cell Shape
Chemistry
Colloidal state and disperse state
Compressive Strength
Elasticity
Exact sciences and technology
General and physical chemistry
Humans
Jurkat Cells
Membranes
Microscopy, Atomic Force - methods
Stress, Mechanical
T-Lymphocytes - cytology
T-Lymphocytes - physiology
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Beschreibung
Zusammenfassung:We report herein the establishment of a single-cell compression method based on force measurements in atomic force microscopy (AFM). The high-resolution bright-field or confocal laser scanning microscopy guides the location of the AFM probe and then monitors the deformation of cell shape, while microsphere-modified AFM probes compress the cell and measure the force. Force and deformation profiles of living cells reveal a cubic relationship at small deformation (
ISSN:0743-7463
1520-5827
DOI:10.1021/la060561p