Identification of large-scale molecular changes of Autotaxin(ENPP2) knock-down by small interfering RNA in breast cancer cells

Identification of large-scale molecular changes of Autotaxin(ENPP2) knock-down by small interfering RNA in breast cancer cells

To understand comprehensive molecular mechanisms by which Autotaxin (ENPP2) mediates, we identified large-scale molecular changes responsible for aberrant expression of Autotaxin (ATX) on breast cancer cells by using DNA microarrays. Transcriptional over-expression of ENPP2 gene was endogenously sil...

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Veröffentlicht in:Molecular and cellular biochemistry 2006-08, Vol.288 (1-2), p.91-106
Hauptverfasser: Noh, Ji Heon, Ryu, So Yeon, Eun, Jung Woo, Song, Jaehwi, Ahn, Young Min, Kim, Su Young, Lee, Sug Hyung, Park, Won Sang, Yoo, Nam Jin, Lee, Jung Young, Lee, Shi Nae, Nam, Suk Woo
Format: Artikel
Sprache:eng
Schlagworte:
Breast Neoplasms - genetics
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Cell Line, Tumor
Female
Humans
Multienzyme Complexes - antagonists & inhibitors
Multienzyme Complexes - genetics
Multienzyme Complexes - metabolism
Oligonucleotide Array Sequence Analysis
Phosphodiesterase I - antagonists & inhibitors
Phosphodiesterase I - genetics
Phosphodiesterase I - metabolism
Phosphoric Diester Hydrolases
Pyrophosphatases - antagonists & inhibitors
Pyrophosphatases - genetics
Pyrophosphatases - metabolism
RNA Interference
RNA, Small Interfering - pharmacology
Time Factors
Transfection
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Zusammenfassung:To understand comprehensive molecular mechanisms by which Autotaxin (ENPP2) mediates, we identified large-scale molecular changes responsible for aberrant expression of Autotaxin (ATX) on breast cancer cells by using DNA microarrays. Transcriptional over-expression of ENPP2 gene was endogenously silenced by using RNA interference technique, and then recapitulated corresponding molecular changes in MDA435 breast cancer cells. Application of nonparametric Wilcoxon statistical analyses (P
ISSN:0300-8177
DOI:10.1007/s11010-006-9124-8