Antisense‐Mediated Knockdown of iNOS Expression in the Presence of Cytokines

The impact of nitric oxide (NO) synthesized after activation by proinflammatory cytokines and/or bacterial products by an inducible NO synthase (iNOS) is still contradictory. Various methods to inhibit iNOS expression or activity have been established. A relatively new approach to inhibit iNOS‐derived NO production is the antisense (AS) technique, which theoretically provides a specific and efficient method for inhibiting gene expression and function. This chapter focusses on the application of iNOS‐specific AS‐oligodeoxynucleotide (ODN) and highlights some of the pitfalls that must be considered to use this technique effectively.