A role for 3- O-sulfotransferase isoform-4 in assisting HSV-1 entry and spread

Many heparan sulfate (HS) 3- O-sulfotransferase (3- OST) isoforms generate cellular receptors for herpes simplex virus type-1 (HSV-1) glycoprotein D (gD). Interestingly, the ability of 3- OST-4 to mediate HSV-1 entry and cell-to-cell fusion has not been determined, although it is predominantly expre...

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Veröffentlicht in:Biochemical and biophysical research communications 2005-12, Vol.338 (2), p.930-937
Hauptverfasser: Tiwari, Vaibhav, O’Donnell, Christopher D., Oh, Myung-Jin, Valyi-Nagy, Tibor, Shukla, Deepak
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Sprache:eng
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Zusammenfassung:Many heparan sulfate (HS) 3- O-sulfotransferase (3- OST) isoforms generate cellular receptors for herpes simplex virus type-1 (HSV-1) glycoprotein D (gD). Interestingly, the ability of 3- OST-4 to mediate HSV-1 entry and cell-to-cell fusion has not been determined, although it is predominantly expressed in the brain, a primary target of HSV-1 infections. We report that expression of 3- OST-4 can render Chinese hamster ovary K1 (CHO-K1) cells susceptible to entry of wild-type and a mutant (Rid1) strain of HSV-1. Evidence for generation of gD receptors by 3- OST-4 was suggested by gD-mediated interference assay and the ability of 3- OST-4 expressing CHO-K1 cells to preferentially bind HSV-1 gD, which could be reversed by prior treatment of cells with HS lyases (heparinases-II/III). In addition, 3- OST-4 expressing CHO-K1 cells acquired the ability to fuse with cells-expressing HSV-1 glycoproteins. Demonstrating specificity, the cell fusion was inhibited by soluble 3-O-sulfated forms of HS, but not unmodified HS. Taken together our results suggest a role of 3- OST-4 in HSV-1 pathogenesis.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2005.10.056