Substrate Distortion by a Lichenase Highlights the Different Conformational Itineraries Harnessed by Related Glycoside Hydrolases

Pick‐a‐path: A series of enzymatic snapshots along the reaction coordinate of a lichenase (specific for gluco‐configured substrates) demonstrates that this enzyme uses a different reaction pathway, featuring a 1S3→4H3→4C1 migration, to that previously observed for the closely related mannanases....

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Veröffentlicht in:	Angewandte Chemie International Edition 2006-08, Vol.45 (31), p.5136-5140
Hauptverfasser:	Money, Victoria A., Smith, Nicola L., Scaffidi, Adrian, Stick, Robert V., Gilbert, Harry J., Davies, Gideon J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Clostridium thermocellum - enzymology drug design enzyme models Glycoside Hydrolases - chemistry Glycoside Hydrolases - classification Glycoside Hydrolases - metabolism glycosides Models, Molecular Protein Conformation reaction pathways Substrate Specificity transition states
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Beschreibung
Zusammenfassung:	Pick‐a‐path: A series of enzymatic snapshots along the reaction coordinate of a lichenase (specific for gluco‐configured substrates) demonstrates that this enzyme uses a different reaction pathway, featuring a 1S3→4H3→4C1 migration, to that previously observed for the closely related mannanases.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.200600802