Nanoscale resolution in GFP-based microscopy

We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The ∼70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results ma...

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Veröffentlicht in:	Nature methods 2006-09, Vol.3 (9), p.721-723
Hauptverfasser:	Willig, Katrin I, Kellner, Robert R, Medda, Rebecca, Hein, Birka, Jakobs, Stefan, Hell, Stefan W
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology brief-communication Cellular biology Diffraction Emissions Endoplasmic Reticulum - ultrastructure Fluorescence Fluorescence microscopy Genetic markers Green Fluorescent Proteins - genetics Humans Imaging, Three-Dimensional Life Sciences Mammals Methods Microscopy Microscopy, Confocal Microscopy, Fluorescence - instrumentation Microscopy, Fluorescence - nursing Microtubules - ultrastructure Nanotechnology Nanovirus Protein Transport Proteomics Viral Proteins - genetics Viral Proteins - metabolism Viruses
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Zusammenfassung:	We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The ∼70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.
ISSN:	1548-7091 1548-7105
DOI:	10.1038/nmeth922