Laser zona drilling does not induce hsp70i transcription in blastomeres of eight-cell mouse embryos
To assess whether zona drilling with a 1,480-nm laser induces heat shock in eight-cell embryos, we measured hsp70i RNA levels in sets of single blastomeres isolated after laser treatment of mouse embryos that had or had not been heated at 43°C. Unlike heating, laser zona drilling did not stimulate h...
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Veröffentlicht in: | Fertility and sterility 2005-11, Vol.84 (5), p.1547-1550 |
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Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
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Online-Zugang: | Volltext |
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Zusammenfassung: | To assess whether zona drilling with a 1,480-nm laser induces heat shock in eight-cell embryos, we measured
hsp70i RNA levels in sets of single blastomeres isolated after laser treatment of mouse embryos that had or had not been heated at 43°C. Unlike heating, laser zona drilling did not stimulate
hsp70i expression, even in the blastomeres closest to the laser beam, corroborating the safety of this procedure for assisted reproduction. |
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ISSN: | 0015-0282 1556-5653 |
DOI: | 10.1016/j.fertnstert.2005.05.023 |