Cloning and expression of canine interferon-alpha genes in Escherichia coli

We cloned five new subtypes of cDNA encoding canine interferon-alpha (CaIFN-alpha) from a canine epithelial cell line. CaIFN-alphas were divided into two groups by amino acid sequences and a molecular phylogenic tree. Two subtypes of them were expressed in Escherichia coli, and IFN proteins were pur...

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Veröffentlicht in:	Journal of Veterinary Medical Science 2005, Vol.67(10), pp.1059-1062
Hauptverfasser:	Taira, O.(Kitasato Univ., Towada, Aomori (Japan). School of Veterinary Medicine and Animal Sciences), Watanugi, I, Hagiwara, Y, Takahashi, M, Arai, S, Sato, H, Maehara, N
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoviruses, Canine Amino Acid Sequence Animals canine IFN-α Cell Line CHIEN CLONACION MOLECULAR CLONAGE MOLECULAIRE cloning Cloning, Molecular - methods Cytopathogenic Effect, Viral DNA Primers DNA, Complementary - genetics DOGS Dogs - genetics ESCHERICHIA COLI EXPRESION GENICA expression EXPRESSION DES GENES GENE EXPRESSION INTERFERON Interferon-alpha - genetics INTERFERONAS INTERFERONS MOLECULAR CLONING Molecular Sequence Data PERRO Phylogeny Sequence Analysis, DNA Sequence Homology Species Specificity Vesicular stomatitis New Jersey virus Vesiculovirus
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Zusammenfassung:	We cloned five new subtypes of cDNA encoding canine interferon-alpha (CaIFN-alpha) from a canine epithelial cell line. CaIFN-alphas were divided into two groups by amino acid sequences and a molecular phylogenic tree. Two subtypes of them were expressed in Escherichia coli, and IFN proteins were purified. Recombinant CaIFN-alphas were highly species-specific and showed antiviral activity against Vesicular stomatitis New Jersey virus and canine adenovirus-1, but not against canine herpesvirus-1.
ISSN:	0916-7250 1347-7439
DOI:	10.1292/jvms.67.1059