Location of the bases modified by M.BcoKIA and M.BcoKIB methylases in the sequence 5 -CTCTTC-3 /5 -GAAGAG-3

The strain Bacillus coagulans K contains two DNA-methyltransferases, M.BcoKIA and M.BcoKIB, which recognize the sequence 5 -CTCTTC-3 /5 -GAAGAG-3 and possess N4-methylcytosine and N6-methyladenine specificities, respectively. A special construct containing the recognition site of BcoKI and sites of...

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Veröffentlicht in:	Biochemistry (Moscow) 2005-10, Vol.70 (10), p.1126-1128
Hauptverfasser:	Svadbina, I V, Matvienko, N N, Zheleznaya, L A, Matvienko, N I
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus (Bacteria) Bacillus coagulans Bacteria Bacteriocins Base Sequence Deoxyribonucleic acid DNA DNA Modification Methylases - chemistry DNA Modification Methylases - metabolism DNA-Cytosine Methylases - chemistry DNA-Cytosine Methylases - metabolism Endonuclease Methyltransferases N6-methyladenosine Nucleotide sequence Nucleotides Site-Specific DNA-Methyltransferase (Adenine-Specific) - chemistry Site-Specific DNA-Methyltransferase (Adenine-Specific) - metabolism Substrate Specificity
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Zusammenfassung:	The strain Bacillus coagulans K contains two DNA-methyltransferases, M.BcoKIA and M.BcoKIB, which recognize the sequence 5 -CTCTTC-3 /5 -GAAGAG-3 and possess N4-methylcytosine and N6-methyladenine specificities, respectively. A special construct containing the recognition site of BcoKI and sites of four IIS restriction endonucleases (IIS restriction endonuclease cassette) was designed to locate the nucleotides modified by the methylases. The modified bases were determined as: 5 -m(4)CTCTTC-3 /5 -GAAGAm(6)G-3 .
ISSN:	0006-2979 1608-3040 0320-9725
DOI:	10.1007/s10541-005-0234-9