Immunization with Major Outer Membrane Protein of Vibrio vulnificus Elicits Protective Antibodies in a Murine Model

Sera from rabbits were infected with Vibrio vulnificus containing an antibody against major outer membrane protein (MOMP). MOMP of V. vulnificus ATCC 27562 were isolated and purified by Sarkosyl and TritonX-100 dual treatment. Molecular size of MOMP was identified as 36-kDa on 13% SDS-PAGE. The sequ...

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Veröffentlicht in:	The journal of microbiology 2005-10, Vol.43 (5), p.437-442
Hauptverfasser:	Jung, C.R. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), Park, M.J. (Korea Institute of Oriental Medicine, Daejeon, Republic of Korea), Heo, M.S. (Cheju National University, Jeju, Republic of Korea), E-mail: msheo@cheju.ac.kr
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Antibodies, Bacterial - blood Bacterial Outer Membrane Proteins - administration & dosage Bacterial Outer Membrane Proteins - chemistry Bacterial Outer Membrane Proteins - immunology Bacterial Vaccines - administration & dosage Bacterial Vaccines - immunology Disease Models, Animal Female Humans immunogenecity Mice Mice, Inbred BALB C Molecular Sequence Data outer membrane protein Rabbits Specific Pathogen-Free Organisms VACCIN Vaccination VACCINES VACUNA Vibrio Infections - immunology Vibrio Infections - prevention & control VIBRIO VULNIFICUS Vibrio vulnificus - immunology
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Zusammenfassung:	Sera from rabbits were infected with Vibrio vulnificus containing an antibody against major outer membrane protein (MOMP). MOMP of V. vulnificus ATCC 27562 were isolated and purified by Sarkosyl and TritonX-100 dual treatment. Molecular size of MOMP was identified as 36-kDa on 13% SDS-PAGE. The sequence of the first 26 amino acid residues from the N-terminal end of the protein is AELYNQDGTSLDMGGRAEARLSMKDG, which is a perfect match with OmpU of V. vulnificus CMCP6 and YJ016. MOMP specific IgM and IgG were investigated in groups of mice.
ISSN:	1225-8873 1976-3794