Genome-wide analysis of phenobarbital-inducible genes in Drosophila melanogaster
An oligoarray analysis was conducted to determine the differential expression of genes due to phenobarbital exposure in Drosophila melanogaster (w¹¹¹⁸ strain) third instar larvae. Seventeen genes were observed to be induced with increased expression by a statistical analysis of microarrays approach...
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Veröffentlicht in: | Insect molecular biology 2006-08, Vol.15 (4), p.455-464 |
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creator | Sun, W Margam, V.M Sun, L Buczkowski, G Bennett, G.W Schemerhorn, B Muir, W.M Pittendrigh, B.R |
description | An oligoarray analysis was conducted to determine the differential expression of genes due to phenobarbital exposure in Drosophila melanogaster (w¹¹¹⁸ strain) third instar larvae. Seventeen genes were observed to be induced with increased expression by a statistical analysis of microarrays approach with a q |
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Seventeen genes were observed to be induced with increased expression by a statistical analysis of microarrays approach with a q <= 0.05. At q <= 0.12, four more genes (Cyp12d1, DmGstd4, and two genes with unknown function) were found to be up-regulated, and 11 genes with unknown function were found to be down-regulated. Fifteen of these genes, Cyp4d14, Cyp6a2, Cyp6a8, Cyp12d1, Cyp6d5, Cyp6w1, CG2065, DmGstd6, DmGstd7, Amy-p/Amy-d, Ugt86Dd, GC5724, Jheh1, Jheh2 and CG11893, were verified using quantitative real time polymerase chain reaction. Some of these genes have been shown to be over-transcribed in metabolically DDT-resistant Drosophila strains.</description><identifier>ISSN: 0962-1075</identifier><identifier>EISSN: 1365-2583</identifier><identifier>DOI: 10.1111/j.1365-2583.2006.00662.x</identifier><identifier>PMID: 16907832</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Animals ; carbohydrate metabolism ; DDT (pesticide) ; differential gene expression ; DNA Primers ; Drosophila melanogaster ; Drosophila melanogaster - enzymology ; Drosophila melanogaster - genetics ; Enzymes - genetics ; Enzymes - metabolism ; gene expression regulation ; Gene Expression Regulation, Enzymologic - drug effects ; gene induction ; Genome - genetics ; GST ; insecticide resistance ; Larva - metabolism ; Microarray Analysis ; microarray technology ; oxido-reductase ; P450 ; phenobarbital ; Phenobarbital - pharmacology ; polymerase chain reaction ; quantitative real time polymerase chain reaction ; transcription (genetics)</subject><ispartof>Insect molecular biology, 2006-08, Vol.15 (4), p.455-464</ispartof><rights>2006 The Royal Entomological Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5212-c1d2bd606c276907b959cf84b499dded8ae99d405cfa0f7104b332c3b87357ea3</citedby><cites>FETCH-LOGICAL-c5212-c1d2bd606c276907b959cf84b499dded8ae99d405cfa0f7104b332c3b87357ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2583.2006.00662.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2583.2006.00662.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16907832$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, W</creatorcontrib><creatorcontrib>Margam, V.M</creatorcontrib><creatorcontrib>Sun, L</creatorcontrib><creatorcontrib>Buczkowski, G</creatorcontrib><creatorcontrib>Bennett, G.W</creatorcontrib><creatorcontrib>Schemerhorn, B</creatorcontrib><creatorcontrib>Muir, W.M</creatorcontrib><creatorcontrib>Pittendrigh, B.R</creatorcontrib><title>Genome-wide analysis of phenobarbital-inducible genes in Drosophila melanogaster</title><title>Insect molecular biology</title><addtitle>Insect Mol Biol</addtitle><description>An oligoarray analysis was conducted to determine the differential expression of genes due to phenobarbital exposure in Drosophila melanogaster (w¹¹¹⁸ strain) third instar larvae. Seventeen genes were observed to be induced with increased expression by a statistical analysis of microarrays approach with a q <= 0.05. At q <= 0.12, four more genes (Cyp12d1, DmGstd4, and two genes with unknown function) were found to be up-regulated, and 11 genes with unknown function were found to be down-regulated. Fifteen of these genes, Cyp4d14, Cyp6a2, Cyp6a8, Cyp12d1, Cyp6d5, Cyp6w1, CG2065, DmGstd6, DmGstd7, Amy-p/Amy-d, Ugt86Dd, GC5724, Jheh1, Jheh2 and CG11893, were verified using quantitative real time polymerase chain reaction. Some of these genes have been shown to be over-transcribed in metabolically DDT-resistant Drosophila strains.</description><subject>Animals</subject><subject>carbohydrate metabolism</subject><subject>DDT (pesticide)</subject><subject>differential gene expression</subject><subject>DNA Primers</subject><subject>Drosophila melanogaster</subject><subject>Drosophila melanogaster - enzymology</subject><subject>Drosophila melanogaster - genetics</subject><subject>Enzymes - genetics</subject><subject>Enzymes - metabolism</subject><subject>gene expression regulation</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>gene induction</subject><subject>Genome - genetics</subject><subject>GST</subject><subject>insecticide resistance</subject><subject>Larva - metabolism</subject><subject>Microarray Analysis</subject><subject>microarray technology</subject><subject>oxido-reductase</subject><subject>P450</subject><subject>phenobarbital</subject><subject>Phenobarbital - pharmacology</subject><subject>polymerase chain reaction</subject><subject>quantitative real time polymerase chain reaction</subject><subject>transcription (genetics)</subject><issn>0962-1075</issn><issn>1365-2583</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkFtv1DAQhS1ERZctfwEiHvqWdGwndiLxQrewLSrlWvVxZCfO1ksui71Rd_99HbIqEk9YGnmkOedo5iMkopDQ8M7WCeUii1mW84QBiCSUYMnuGZk9DZ6TGRSCxRRkdkxeer8GgLwQxQtyTEUBMudsRr4uTde3Jn6wlYlUp5q9tz7q62hzHwZaOW23qoltVw2l1Y2JVqYzPrJddOF632_ubaOi1jSq61fKb407IUe1arx5dfjn5Pbjh5-Ly_j6y_Jq8f46LjNGWVzSiulKgCiZHJfRRVaUdZ7qtCiqylS5MqFJIStrBbWkkGrOWcl1LnkmjeJzcjrlblz_ezB-i631pWnCJqYfPIpcZgGKCMK3_wjX_eDCpR4ZpJIJGSLnJJ9EZbjKO1PjxtlWuT1SwBE5rnEkiyNZHJHjH-S4C9bXh_xBt6b6azwwDoJ3k-DBNmb_38F49fk8NMEeT3Yb8O6e7Mr9QiG5zPDuZol357C4-JR-w-9B_2bS16pHtXLW4-0PBpQDhYKlKeOPOrSnaQ</recordid><startdate>200608</startdate><enddate>200608</enddate><creator>Sun, W</creator><creator>Margam, V.M</creator><creator>Sun, L</creator><creator>Buczkowski, G</creator><creator>Bennett, G.W</creator><creator>Schemerhorn, B</creator><creator>Muir, W.M</creator><creator>Pittendrigh, B.R</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200608</creationdate><title>Genome-wide analysis of phenobarbital-inducible genes in Drosophila melanogaster</title><author>Sun, W ; Margam, V.M ; Sun, L ; Buczkowski, G ; Bennett, G.W ; Schemerhorn, B ; Muir, W.M ; Pittendrigh, B.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5212-c1d2bd606c276907b959cf84b499dded8ae99d405cfa0f7104b332c3b87357ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>carbohydrate metabolism</topic><topic>DDT (pesticide)</topic><topic>differential gene expression</topic><topic>DNA Primers</topic><topic>Drosophila melanogaster</topic><topic>Drosophila melanogaster - enzymology</topic><topic>Drosophila melanogaster - genetics</topic><topic>Enzymes - genetics</topic><topic>Enzymes - metabolism</topic><topic>gene expression regulation</topic><topic>Gene Expression Regulation, Enzymologic - drug effects</topic><topic>gene induction</topic><topic>Genome - genetics</topic><topic>GST</topic><topic>insecticide resistance</topic><topic>Larva - metabolism</topic><topic>Microarray Analysis</topic><topic>microarray technology</topic><topic>oxido-reductase</topic><topic>P450</topic><topic>phenobarbital</topic><topic>Phenobarbital - pharmacology</topic><topic>polymerase chain reaction</topic><topic>quantitative real time polymerase chain reaction</topic><topic>transcription (genetics)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, W</creatorcontrib><creatorcontrib>Margam, V.M</creatorcontrib><creatorcontrib>Sun, L</creatorcontrib><creatorcontrib>Buczkowski, G</creatorcontrib><creatorcontrib>Bennett, G.W</creatorcontrib><creatorcontrib>Schemerhorn, B</creatorcontrib><creatorcontrib>Muir, W.M</creatorcontrib><creatorcontrib>Pittendrigh, B.R</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Insect molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, W</au><au>Margam, V.M</au><au>Sun, L</au><au>Buczkowski, G</au><au>Bennett, G.W</au><au>Schemerhorn, B</au><au>Muir, W.M</au><au>Pittendrigh, B.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome-wide analysis of phenobarbital-inducible genes in Drosophila melanogaster</atitle><jtitle>Insect molecular biology</jtitle><addtitle>Insect Mol Biol</addtitle><date>2006-08</date><risdate>2006</risdate><volume>15</volume><issue>4</issue><spage>455</spage><epage>464</epage><pages>455-464</pages><issn>0962-1075</issn><eissn>1365-2583</eissn><abstract>An oligoarray analysis was conducted to determine the differential expression of genes due to phenobarbital exposure in Drosophila melanogaster (w¹¹¹⁸ strain) third instar larvae. Seventeen genes were observed to be induced with increased expression by a statistical analysis of microarrays approach with a q <= 0.05. At q <= 0.12, four more genes (Cyp12d1, DmGstd4, and two genes with unknown function) were found to be up-regulated, and 11 genes with unknown function were found to be down-regulated. Fifteen of these genes, Cyp4d14, Cyp6a2, Cyp6a8, Cyp12d1, Cyp6d5, Cyp6w1, CG2065, DmGstd6, DmGstd7, Amy-p/Amy-d, Ugt86Dd, GC5724, Jheh1, Jheh2 and CG11893, were verified using quantitative real time polymerase chain reaction. Some of these genes have been shown to be over-transcribed in metabolically DDT-resistant Drosophila strains.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>16907832</pmid><doi>10.1111/j.1365-2583.2006.00662.x</doi><tpages>10</tpages></addata></record> |
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subjects | Animals carbohydrate metabolism DDT (pesticide) differential gene expression DNA Primers Drosophila melanogaster Drosophila melanogaster - enzymology Drosophila melanogaster - genetics Enzymes - genetics Enzymes - metabolism gene expression regulation Gene Expression Regulation, Enzymologic - drug effects gene induction Genome - genetics GST insecticide resistance Larva - metabolism Microarray Analysis microarray technology oxido-reductase P450 phenobarbital Phenobarbital - pharmacology polymerase chain reaction quantitative real time polymerase chain reaction transcription (genetics) |
title | Genome-wide analysis of phenobarbital-inducible genes in Drosophila melanogaster |
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