Detection of Burkholderia cepacia DNA from Artificially Infected EDTA-blood and Lung Tissue Comparing Different DNA Isolation Methods

Bacterial DNA (Burkholderia cepacia) was prepared from artificially infected equine ethylenediaminetetraacetic acid (EDTA)-blood and lung tissue by using four standard methods (lysis buffer containing proteinase K, phenol/chloroform/isoamylalcohol-extraction, microwave-treatment, heat treatment) and six commercially available kits (Puregene®, High Pure PCR Template Preparation Kit®, InstaGene®, QiaAmp Tissue Kit®, DNAzol® and Elu-Quik®). After a subsequent polymerase chain reaction (PCR), their efficacy and sensitivity were compared. Concerning the detection limits, the simple lysis with a proteinase K-containing buffer led to the best results for EDTA-blood as well as for artificially infected lung tissue.