Consumption of milk from transgenic goats expressing human lysozyme in the mammary gland results in the modulation of intestinal microflora
Lysozyme is a key antimicrobial component of human milk that has several health-promoting functions including the development of a healthy intestinal tract. However, levels of lysozyme in the milk of dairy animals are negligible. We have generated transgenic dairy goats that express human lysozyme (...
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| Veröffentlicht in: | Transgenic research 2006-08, Vol.15 (4), p.515-519 |
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| creator | MAGA, Elizabeth A WALKER, Richard L ANDERSON, Gary B MURRAY, James D |
| description | Lysozyme is a key antimicrobial component of human milk that has several health-promoting functions including the development of a healthy intestinal tract. However, levels of lysozyme in the milk of dairy animals are negligible. We have generated transgenic dairy goats that express human lysozyme (HLZ) in their milk in an attempt to deliver the benefits of human milk in a continual fashion. To test the feasibility of this transgenic approach to achieve a biological impact at the level of the intestine, feeding trials were conducted in two animal models. Pasteurized milk from HLZ transgenic animals was fed to both kid goats (ruminant model) and young pigs (human model), and the numbers of total coliforms and Escherichia coli present in the small intestine were determined. Data from this proof-of-principle study demonstrate that milk from transgenic animals was capable of modulating the bacterial population of the gut in both animal models. Pigs that consumed pasteurized milk from HLZ transgenic goats had fewer numbers of coliforms and E. coli in their intestine than did those receiving milk from non-transgenic control animals. The opposite effect was seen in goats. Milk from these transgenic animals not only represent one of the first transgenic food products with the potential of benefiting human health, but are also a unique model to study the development and role of intestinal microflora on health, well-being and resistance to disease. |
| doi_str_mv | 10.1007/s11248-006-0014-3 |
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However, levels of lysozyme in the milk of dairy animals are negligible. We have generated transgenic dairy goats that express human lysozyme (HLZ) in their milk in an attempt to deliver the benefits of human milk in a continual fashion. To test the feasibility of this transgenic approach to achieve a biological impact at the level of the intestine, feeding trials were conducted in two animal models. Pasteurized milk from HLZ transgenic animals was fed to both kid goats (ruminant model) and young pigs (human model), and the numbers of total coliforms and Escherichia coli present in the small intestine were determined. Data from this proof-of-principle study demonstrate that milk from transgenic animals was capable of modulating the bacterial population of the gut in both animal models. Pigs that consumed pasteurized milk from HLZ transgenic goats had fewer numbers of coliforms and E. coli in their intestine than did those receiving milk from non-transgenic control animals. The opposite effect was seen in goats. Milk from these transgenic animals not only represent one of the first transgenic food products with the potential of benefiting human health, but are also a unique model to study the development and role of intestinal microflora on health, well-being and resistance to disease.</description><identifier>ISSN: 0962-8819</identifier><identifier>EISSN: 1573-9368</identifier><identifier>DOI: 10.1007/s11248-006-0014-3</identifier><identifier>PMID: 16906451</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Animal models ; Animals ; Animals, Genetically Modified ; Bacteria ; Biological and medical sciences ; Biotechnology ; Body Weight ; Breast milk ; Coliforms ; Consumption ; Diet ; Disease resistance ; E coli ; Escherichia coli ; Feeding trials ; Female ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; Goats ; Humans ; Intestinal microflora ; Intestine, Small - microbiology ; Lysozyme ; Male ; Mammary gland ; Mammary Glands, Animal - metabolism ; Methods. Procedures. Technologies ; Microflora ; Milk ; Milk Proteins - chemistry ; Milk, Human ; Muramidase - genetics ; Pasteurized milk ; Ruminantia ; Small intestine ; Swine ; Time Factors ; Transgenic animals ; Transgenic animals and transgenic plants</subject><ispartof>Transgenic research, 2006-08, Vol.15 (4), p.515-519</ispartof><rights>2006 INIST-CNRS</rights><rights>Springer Science+Business Media B.V. 2006.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-c5cc52123301b0e2a9a8b8eadd28dd72377d50f78652ada4ea7a04f99b7bde933</citedby><cites>FETCH-LOGICAL-c453t-c5cc52123301b0e2a9a8b8eadd28dd72377d50f78652ada4ea7a04f99b7bde933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27928,27929</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18058996$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16906451$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MAGA, Elizabeth A</creatorcontrib><creatorcontrib>WALKER, Richard L</creatorcontrib><creatorcontrib>ANDERSON, Gary B</creatorcontrib><creatorcontrib>MURRAY, James D</creatorcontrib><title>Consumption of milk from transgenic goats expressing human lysozyme in the mammary gland results in the modulation of intestinal microflora</title><title>Transgenic research</title><addtitle>Transgenic Res</addtitle><description>Lysozyme is a key antimicrobial component of human milk that has several health-promoting functions including the development of a healthy intestinal tract. However, levels of lysozyme in the milk of dairy animals are negligible. We have generated transgenic dairy goats that express human lysozyme (HLZ) in their milk in an attempt to deliver the benefits of human milk in a continual fashion. To test the feasibility of this transgenic approach to achieve a biological impact at the level of the intestine, feeding trials were conducted in two animal models. Pasteurized milk from HLZ transgenic animals was fed to both kid goats (ruminant model) and young pigs (human model), and the numbers of total coliforms and Escherichia coli present in the small intestine were determined. Data from this proof-of-principle study demonstrate that milk from transgenic animals was capable of modulating the bacterial population of the gut in both animal models. Pigs that consumed pasteurized milk from HLZ transgenic goats had fewer numbers of coliforms and E. coli in their intestine than did those receiving milk from non-transgenic control animals. The opposite effect was seen in goats. Milk from these transgenic animals not only represent one of the first transgenic food products with the potential of benefiting human health, but are also a unique model to study the development and role of intestinal microflora on health, well-being and resistance to disease.</description><subject>Animal models</subject><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Body Weight</subject><subject>Breast milk</subject><subject>Coliforms</subject><subject>Consumption</subject><subject>Diet</subject><subject>Disease resistance</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Feeding trials</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Goats</subject><subject>Humans</subject><subject>Intestinal microflora</subject><subject>Intestine, Small - microbiology</subject><subject>Lysozyme</subject><subject>Male</subject><subject>Mammary gland</subject><subject>Mammary Glands, Animal - metabolism</subject><subject>Methods. Procedures. Technologies</subject><subject>Microflora</subject><subject>Milk</subject><subject>Milk Proteins - chemistry</subject><subject>Milk, Human</subject><subject>Muramidase - genetics</subject><subject>Pasteurized milk</subject><subject>Ruminantia</subject><subject>Small intestine</subject><subject>Swine</subject><subject>Time Factors</subject><subject>Transgenic animals</subject><subject>Transgenic animals and transgenic plants</subject><issn>0962-8819</issn><issn>1573-9368</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkcuKFDEUhoMoTs_oA7iRIDi70lwqlWQpjTcYcKPrcKqS6smYS5tUwbSv4EubpnsYcOMiZJHvnPOffAi9ouQdJUS-r5SyXnWEDO3QvuNP0IYKyTvNB_UUbYgeWKcU1Rfosta7xhCi-HN0QQdNhl7QDfqzzamucb_4nHCecfThJ55LjngpkOrOJT_hXYalYne_L65Wn3b4do2QcDjU_PsQHfYJL7cOR4gRygHvAiSLG7uGVvbwmO0a4GGMT4uri08Q2sSp5DnkAi_QsxlCdS_P9xX68enj9-2X7ubb56_bDzfd1Au-dJOYJsEo45zQkTgGGtSoHFjLlLWScSmtILNUg2BgoXcggfSz1qMcrdOcX6HrU999yb_WlsNEXycXWmyX12oGJXumuPgvSLWUUpChgW_-Ae_yWtp21UjBCNGy1w2iJ6jtW2txs9kXf_wwQ4k5-jQnn6b5NEef5hj19bnxOkZnHyvOAhvw9gxAnSDMTdrk6yOniFBaD_wvzGmrTQ</recordid><startdate>20060801</startdate><enddate>20060801</enddate><creator>MAGA, Elizabeth A</creator><creator>WALKER, Richard L</creator><creator>ANDERSON, Gary B</creator><creator>MURRAY, James D</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7QL</scope><scope>7QO</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20060801</creationdate><title>Consumption of milk from transgenic goats expressing human lysozyme in the mammary gland results in the modulation of intestinal microflora</title><author>MAGA, Elizabeth A ; WALKER, Richard L ; ANDERSON, Gary B ; MURRAY, James D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-c5cc52123301b0e2a9a8b8eadd28dd72377d50f78652ada4ea7a04f99b7bde933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animal models</topic><topic>Animals</topic><topic>Animals, Genetically Modified</topic><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Body Weight</topic><topic>Breast milk</topic><topic>Coliforms</topic><topic>Consumption</topic><topic>Diet</topic><topic>Disease resistance</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Feeding trials</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Goats</topic><topic>Humans</topic><topic>Intestinal microflora</topic><topic>Intestine, Small - microbiology</topic><topic>Lysozyme</topic><topic>Male</topic><topic>Mammary gland</topic><topic>Mammary Glands, Animal - metabolism</topic><topic>Methods. Procedures. Technologies</topic><topic>Microflora</topic><topic>Milk</topic><topic>Milk Proteins - chemistry</topic><topic>Milk, Human</topic><topic>Muramidase - genetics</topic><topic>Pasteurized milk</topic><topic>Ruminantia</topic><topic>Small intestine</topic><topic>Swine</topic><topic>Time Factors</topic><topic>Transgenic animals</topic><topic>Transgenic animals and transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MAGA, Elizabeth A</creatorcontrib><creatorcontrib>WALKER, Richard L</creatorcontrib><creatorcontrib>ANDERSON, Gary B</creatorcontrib><creatorcontrib>MURRAY, James D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Transgenic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MAGA, Elizabeth A</au><au>WALKER, Richard L</au><au>ANDERSON, Gary B</au><au>MURRAY, James D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Consumption of milk from transgenic goats expressing human lysozyme in the mammary gland results in the modulation of intestinal microflora</atitle><jtitle>Transgenic research</jtitle><addtitle>Transgenic Res</addtitle><date>2006-08-01</date><risdate>2006</risdate><volume>15</volume><issue>4</issue><spage>515</spage><epage>519</epage><pages>515-519</pages><issn>0962-8819</issn><eissn>1573-9368</eissn><abstract>Lysozyme is a key antimicrobial component of human milk that has several health-promoting functions including the development of a healthy intestinal tract. However, levels of lysozyme in the milk of dairy animals are negligible. We have generated transgenic dairy goats that express human lysozyme (HLZ) in their milk in an attempt to deliver the benefits of human milk in a continual fashion. To test the feasibility of this transgenic approach to achieve a biological impact at the level of the intestine, feeding trials were conducted in two animal models. Pasteurized milk from HLZ transgenic animals was fed to both kid goats (ruminant model) and young pigs (human model), and the numbers of total coliforms and Escherichia coli present in the small intestine were determined. Data from this proof-of-principle study demonstrate that milk from transgenic animals was capable of modulating the bacterial population of the gut in both animal models. Pigs that consumed pasteurized milk from HLZ transgenic goats had fewer numbers of coliforms and E. coli in their intestine than did those receiving milk from non-transgenic control animals. The opposite effect was seen in goats. Milk from these transgenic animals not only represent one of the first transgenic food products with the potential of benefiting human health, but are also a unique model to study the development and role of intestinal microflora on health, well-being and resistance to disease.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>16906451</pmid><doi>10.1007/s11248-006-0014-3</doi><tpages>5</tpages></addata></record> |
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| subjects | Animal models Animals Animals, Genetically Modified Bacteria Biological and medical sciences Biotechnology Body Weight Breast milk Coliforms Consumption Diet Disease resistance E coli Escherichia coli Feeding trials Female Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Goats Humans Intestinal microflora Intestine, Small - microbiology Lysozyme Male Mammary gland Mammary Glands, Animal - metabolism Methods. Procedures. Technologies Microflora Milk Milk Proteins - chemistry Milk, Human Muramidase - genetics Pasteurized milk Ruminantia Small intestine Swine Time Factors Transgenic animals Transgenic animals and transgenic plants |
| title | Consumption of milk from transgenic goats expressing human lysozyme in the mammary gland results in the modulation of intestinal microflora |
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