Isolation and characterization of four antibacterial peptides from bovine hemoglobin

Peptic digestion of bovine hemoglobin at low degree of hydrolysis yields several intermediate peptide fractions after separation by reversed phase HPLC exhibiting antibacterial activity against Micrococcus luteus A270, Listeria innocua, Escherichia coli, and Salmonella enteritidis. From these fracti...

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Veröffentlicht in:	Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2006-09, Vol.27 (9), p.2082-2089
Hauptverfasser:	Nedjar-Arroume, Naima, Dubois-Delval, Véronique, Miloudi, Khalil, Daoud, Rachid, Krier, François, Kouach, Mostafa, Briand, Gilbert, Guillochon, Didier
Format:	Artikel
Sprache:	eng
Schlagworte:	Active peptides Amino Acid Sequence Animals Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - isolation & purification Anti-Bacterial Agents - pharmacology Antibacterial peptides Biological and medical sciences Bovine hemoglobin Cattle Endorphins - chemistry Endorphins - isolation & purification Endorphins - pharmacology Escherichia coli Fundamental and applied biological sciences. Psychology Hemoglobins - chemistry Hemoglobins - isolation & purification Hemoglobins - pharmacology Hydrolysate Hydrolysis Listeria innocua Mass Spectrometry Microbial Sensitivity Tests Micrococcus luteus Molecular Sequence Data Neokyotorphin Pepsin Peptide Fragments - chemistry Peptide Fragments - isolation & purification Peptide Fragments - pharmacology Salmonella enteritidis Vertebrates: endocrinology
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Zusammenfassung:	Peptic digestion of bovine hemoglobin at low degree of hydrolysis yields several intermediate peptide fractions after separation by reversed phase HPLC exhibiting antibacterial activity against Micrococcus luteus A270, Listeria innocua, Escherichia coli, and Salmonella enteritidis. From these fractions, four new antibacterial peptides were isolated and analyzed by ESI-MS/MS. Three of these peptides correspond to fragments of the α-chain of bovine hemoglobin: α107–141, α137–141, and α133–141, and one peptide to the β-chain: β126–145. The minimum inhibitory concentrations (MIC) of these peptides towards the four strains and their hemolytic activity towards bovine erythrocytes were determined.
ISSN:	0196-9781 1873-5169
DOI:	10.1016/j.peptides.2006.03.033