Molecular analysis of a California strain of Rupestris stem pitting-associated virus isolated from declining Syrah grapevines

The sequence of the genome of a Rupestris stem pitting-associated virus (RSPaV) isolated from a declining Syrah grapevine in California, designated the Syrah strain (RSPaV-SY) was determined. The genome of this strain had an overall nucleotide identity of 77% in comparison with RSPaV sequences in Ge...

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Veröffentlicht in:	Archives of virology 2006-09, Vol.151 (9), p.1889-1894
Hauptverfasser:	Lima, M. F, Alkowni, R, Uyemoto, J. K, Golino, D, Osman, F, Rowhani, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences California Capsid Proteins - genetics Cloning Cluster Analysis Design Disease Fundamental and applied biological sciences. Psychology Genome, Viral Genomes Microbiology Miscellaneous Molecular Sequence Data Phylogenetics Phylogeny Proteins RNA Replicase - genetics RNA Viruses - classification RNA Viruses - genetics RNA Viruses - isolation & purification RNA, Viral - genetics Sequence Homology, Nucleic Acid Virology Viruses Vitis - virology Wines
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Beschreibung
Zusammenfassung:	The sequence of the genome of a Rupestris stem pitting-associated virus (RSPaV) isolated from a declining Syrah grapevine in California, designated the Syrah strain (RSPaV-SY) was determined. The genome of this strain had an overall nucleotide identity of 77% in comparison with RSPaV sequences in GenBank; the coat protein was the most conserved gene among RSPaV sequences and the replicase was the least conserved gene. Phylogenetic analysis of partial coat protein and replicase gene sequences showed RSPaV-SY clustered independently from the majority of RSPaV isolates.
ISSN:	0304-8608 1432-8798
DOI:	10.1007/s00705-006-0742-y