Nucleic Acid with Guanidinium Modification Exhibits Efficient Cellular Uptake

Quick on the uptake: A 20‐mer DNA oligomer modified with 5‐[(6‐guanidiniohexylcarbamoyl)methyl]‐2′‐deoxyuridine (Tg) shows efficient cellular uptake (blue arrow) in HeLa cells without any other reagents. The fluorescence microscopy image reveals FAM‐labeled DNA oligomer (green) and stained nuclei (b...

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Veröffentlicht in:	Angewandte Chemie International Edition 2005-10, Vol.44 (41), p.6682-6685
Hauptverfasser:	Ohmichi, Tatsuo, Kuwahara, Masayasu, Sasaki, Naoko, Hasegawa, Masatoshi, Nishikata, Takahito, Sawai, Hiroaki, Sugimoto, Naoki
Format:	Artikel
Sprache:	eng
Schlagworte:	biotechnology cellular uptake Deoxyuridine - analogs & derivatives Deoxyuridine - pharmacokinetics DNA - chemistry DNA - pharmacokinetics Gene Transfer Techniques Guanidine - analogs & derivatives Guanidine - chemistry HeLa Cells Humans Molecular Structure nucleic acids Nucleic Acids - chemistry Nucleic Acids - pharmacokinetics nucleosides oligonucleotides
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Beschreibung
Zusammenfassung:	Quick on the uptake: A 20‐mer DNA oligomer modified with 5‐[(6‐guanidiniohexylcarbamoyl)methyl]‐2′‐deoxyuridine (Tg) shows efficient cellular uptake (blue arrow) in HeLa cells without any other reagents. The fluorescence microscopy image reveals FAM‐labeled DNA oligomer (green) and stained nuclei (blue). FAM=6‐carboxyfluorescein.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.200500904