The β-Glucuronidase Klotho Hydrolyzes and Activates the TRPV5 Channel

The β-Glucuronidase Klotho Hydrolyzes and Activates the TRPV5 Channel

Blood calcium concentration is maintained within a narrow range despite large variations in dietary input and body demand. The Transient Receptor Potential ion channel TRPV5 has been implicated in this process. We report here that TRPV5 is stimulated by the mammalian hormone klotho. Klotho, a β-gluc...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 2005-10, Vol.310 (5747), p.490-493
Hauptverfasser: Chang, Q., Hoefs, S., van der Kemp, A. W., Topala, C. N., Bindels, R. J., Hoenderop, J. G.
Format: Artikel
Sprache:eng
Schlagworte:
Analysis
Animals
Biological and medical sciences
Bones
Calcium - metabolism
Calcium Channels - genetics
Calcium Channels - metabolism
Cell culture techniques
Cell Line
Cell Membrane - metabolism
Cell membranes
Cell membranes. Ionic channels. Membrane pores
Cell structures and functions
Cells, Cultured
Fundamental and applied biological sciences. Psychology
Glucuronidase - antagonists & inhibitors
Glucuronidase - metabolism
Glycosylation
HEK293 cells
Hormones
Humans
Hydrolysis
Ions
Kidney - cytology
Kidney - metabolism
Kidney cells
Kidneys
Membrane Proteins - metabolism
Mice
Mice, Inbred C57BL
Molecular and cellular biology
Mutation
Patch-Clamp Techniques
Protein Transport
Rabbits
Sodium - metabolism
T lymphocytes
Transfection
TRPV Cation Channels - genetics
TRPV Cation Channels - metabolism
Vitamin D
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Zusammenfassung:Blood calcium concentration is maintained within a narrow range despite large variations in dietary input and body demand. The Transient Receptor Potential ion channel TRPV5 has been implicated in this process. We report here that TRPV5 is stimulated by the mammalian hormone klotho. Klotho, a β-glucuronidase, hydrolyzes extracellular sugar residues on TRPV5, entrapping the channel in the plasma membrane. This maintains durable calcium channel activity and membrane calcium permeability in kidney. Thus, klotho activates a cell surface channel by hydrolysis of its extracellular N-linked oligosaccharides.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1114245