Effects of VIP and VIP-DAP on Proliferation and Lipid Peroxidation Metabolism in Human KB Cells

: In the present study, we have utilized the transglutaminase (TGase) enzyme to modify the primary structure of VIP with diamminopropane (DAP) at the level of the Gln16. We have investigated the conformational stability of VIP and VIP–DAP in solution by limited proteolysis experiments. The VIP–DAP...

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Veröffentlicht in:	Annals of the New York Academy of Sciences 2006-07, Vol.1070 (1), p.167-172
Hauptverfasser:	CARAGLIA, MICHELE, DICITORE, ALESSANDRA, GIUBERTI, GAIA, CASSESE, DIANA, LEPRETTI, MARILENA, CARTENÌ, MARIA, ABBRUZZESE, ALBERTO, STIUSO, PAOLA
Format:	Artikel
Sprache:	eng
Schlagworte:	cell cycle modulation Cell Line, Tumor Cell Proliferation - drug effects conformational analysis Diamines - pharmacology Humans intracellular lipid peroxidation limited proteolysis Lipid Metabolism - drug effects Lipid Peroxidation - drug effects vasoactive intestinal peptide Vasoactive Intestinal Peptide - pharmacology vasoactive intestinal peptide analogs
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Beschreibung
Zusammenfassung:	: In the present study, we have utilized the transglutaminase (TGase) enzyme to modify the primary structure of VIP with diamminopropane (DAP) at the level of the Gln16. We have investigated the conformational stability of VIP and VIP–DAP in solution by limited proteolysis experiments. The VIP–DAP appears to be more resistant to the proteolytic attack of trypsin, thus indicating that the derivatization in position 16 is able to stabilize the structure of the peptide. However, we have studied their role in cell cycle modulation and antioxidant activity in the oropharyngeal epidermoid carcinoma KB cells.
ISSN:	0077-8923 1749-6632
DOI:	10.1196/annals.1317.007