Chemical Primer Extension: Efficiently Determining Single Nucleotides in DNA
Rapid replication: Non‐enzymatic primer extension has previously been studied in the context of prebiotic chemistry, but not for practical applications. Reactions with primers featuring a 3′‐amino‐2′,3′‐dideoxynucleotide can be rapid and selective for all four templating nucleobases (see scheme). On...
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| Veröffentlicht in: | Angewandte Chemie International Edition 2005-10, Vol.44 (40), p.6588-6592 |
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| container_end_page | 6592 |
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| container_issue | 40 |
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| container_title | Angewandte Chemie International Edition |
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| creator | Hagenbuch, Patrizia Kervio, Eric Hochgesand, Annette Plutowski, Ulrich Richert, Clemens |
| description | Rapid replication: Non‐enzymatic primer extension has previously been studied in the context of prebiotic chemistry, but not for practical applications. Reactions with primers featuring a 3′‐amino‐2′,3′‐dideoxynucleotide can be rapid and selective for all four templating nucleobases (see scheme). On a chip with immobilized capture strands, 500 fmol of template suffice for single‐nucleotide determinations within 2.7 h. |
| doi_str_mv | 10.1002/anie.200501794 |
| format | Article |
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Reactions with primers featuring a 3′‐amino‐2′,3′‐dideoxynucleotide can be rapid and selective for all four templating nucleobases (see scheme). On a chip with immobilized capture strands, 500 fmol of template suffice for single‐nucleotide determinations within 2.7 h.</description><identifier>ISSN: 1433-7851</identifier><identifier>EISSN: 1521-3773</identifier><identifier>DOI: 10.1002/anie.200501794</identifier><identifier>PMID: 16175646</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Base Sequence ; DNA - chemical synthesis ; DNA - chemistry ; DNA Primers - chemistry ; DNA recognition ; Molecular Sequence Data ; non-enzymatic replication ; Nucleic Acid Amplification Techniques - methods ; Nucleotides - analysis ; Nucleotides - chemistry ; Oligonucleotide Array Sequence Analysis - methods ; oligonucleotides ; primer extension ; single-nucleotide polymorphisms ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Time Factors</subject><ispartof>Angewandte Chemie International Edition, 2005-10, Vol.44 (40), p.6588-6592</ispartof><rights>Copyright © 2005 WILEY‐VCH Verlag GmbH & Co. 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Reactions with primers featuring a 3′‐amino‐2′,3′‐dideoxynucleotide can be rapid and selective for all four templating nucleobases (see scheme). On a chip with immobilized capture strands, 500 fmol of template suffice for single‐nucleotide determinations within 2.7 h.</description><subject>Base Sequence</subject><subject>DNA - chemical synthesis</subject><subject>DNA - chemistry</subject><subject>DNA Primers - chemistry</subject><subject>DNA recognition</subject><subject>Molecular Sequence Data</subject><subject>non-enzymatic replication</subject><subject>Nucleic Acid Amplification Techniques - methods</subject><subject>Nucleotides - analysis</subject><subject>Nucleotides - chemistry</subject><subject>Oligonucleotide Array Sequence Analysis - methods</subject><subject>oligonucleotides</subject><subject>primer extension</subject><subject>single-nucleotide polymorphisms</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>Time Factors</subject><issn>1433-7851</issn><issn>1521-3773</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1PwkAQhjdGI4pePZqevBX3o91tvSEgEhFNIPG42W6nutoP7LYR_r1LIOiNy8wcnvdN5kHoiuAewZjeqtJAj2IcYiLi4AidkZASnwnBjt0dMOaLKCQddG7tp-OjCPNT1CGciJAH_AxNBx9QGK1y77U2BdTeaNVAaU1V3nmjLDPaQNnka28IDdSFKU357s3dyMGbtTqHqjEpWM-U3nDWv0AnmcotXO52Fy0eRovBoz99GU8G_amvA0IDP1EaU5xiJgjWhAeM80QzDGmiBOVxpmKc8FRvHtJKaZ2BylgCFOKIUZqyLrrZ1i7r6rsF28jCWA15rkqoWit5xOMwJtFB0EmInBHqwN4W1HVlbQ2ZXDobql5LguXGs9x4lnvPLnC9a26TAtI_fCfWAfEW-DE5rA_Uyf5sMvpf7m-zxjaw2mdV_SW5YCKUb7OxnJN7Mniaz-Uz-wX9zpkQ</recordid><startdate>20051014</startdate><enddate>20051014</enddate><creator>Hagenbuch, Patrizia</creator><creator>Kervio, Eric</creator><creator>Hochgesand, Annette</creator><creator>Plutowski, Ulrich</creator><creator>Richert, Clemens</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20051014</creationdate><title>Chemical Primer Extension: Efficiently Determining Single Nucleotides in DNA</title><author>Hagenbuch, Patrizia ; Kervio, Eric ; Hochgesand, Annette ; Plutowski, Ulrich ; Richert, Clemens</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4124-bac020d03710c164366bc30edba7269fa90b6dc1794caaccfeaf3be2e98322d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Base Sequence</topic><topic>DNA - chemical synthesis</topic><topic>DNA - chemistry</topic><topic>DNA Primers - chemistry</topic><topic>DNA recognition</topic><topic>Molecular Sequence Data</topic><topic>non-enzymatic replication</topic><topic>Nucleic Acid Amplification Techniques - methods</topic><topic>Nucleotides - analysis</topic><topic>Nucleotides - chemistry</topic><topic>Oligonucleotide Array Sequence Analysis - methods</topic><topic>oligonucleotides</topic><topic>primer extension</topic><topic>single-nucleotide polymorphisms</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hagenbuch, Patrizia</creatorcontrib><creatorcontrib>Kervio, Eric</creatorcontrib><creatorcontrib>Hochgesand, Annette</creatorcontrib><creatorcontrib>Plutowski, Ulrich</creatorcontrib><creatorcontrib>Richert, Clemens</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Angewandte Chemie International Edition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hagenbuch, Patrizia</au><au>Kervio, Eric</au><au>Hochgesand, Annette</au><au>Plutowski, Ulrich</au><au>Richert, Clemens</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chemical Primer Extension: Efficiently Determining Single Nucleotides in DNA</atitle><jtitle>Angewandte Chemie International Edition</jtitle><addtitle>Angewandte Chemie International Edition</addtitle><date>2005-10-14</date><risdate>2005</risdate><volume>44</volume><issue>40</issue><spage>6588</spage><epage>6592</epage><pages>6588-6592</pages><issn>1433-7851</issn><eissn>1521-3773</eissn><abstract>Rapid replication: Non‐enzymatic primer extension has previously been studied in the context of prebiotic chemistry, but not for practical applications. Reactions with primers featuring a 3′‐amino‐2′,3′‐dideoxynucleotide can be rapid and selective for all four templating nucleobases (see scheme). On a chip with immobilized capture strands, 500 fmol of template suffice for single‐nucleotide determinations within 2.7 h.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>16175646</pmid><doi>10.1002/anie.200501794</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Base Sequence DNA - chemical synthesis DNA - chemistry DNA Primers - chemistry DNA recognition Molecular Sequence Data non-enzymatic replication Nucleic Acid Amplification Techniques - methods Nucleotides - analysis Nucleotides - chemistry Oligonucleotide Array Sequence Analysis - methods oligonucleotides primer extension single-nucleotide polymorphisms Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Time Factors |
| title | Chemical Primer Extension: Efficiently Determining Single Nucleotides in DNA |
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