Chemical Primer Extension: Efficiently Determining Single Nucleotides in DNA
Rapid replication: Non‐enzymatic primer extension has previously been studied in the context of prebiotic chemistry, but not for practical applications. Reactions with primers featuring a 3′‐amino‐2′,3′‐dideoxynucleotide can be rapid and selective for all four templating nucleobases (see scheme). On...
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Veröffentlicht in: | Angewandte Chemie International Edition 2005-10, Vol.44 (40), p.6588-6592 |
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Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Rapid replication: Non‐enzymatic primer extension has previously been studied in the context of prebiotic chemistry, but not for practical applications. Reactions with primers featuring a 3′‐amino‐2′,3′‐dideoxynucleotide can be rapid and selective for all four templating nucleobases (see scheme). On a chip with immobilized capture strands, 500 fmol of template suffice for single‐nucleotide determinations within 2.7 h. |
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ISSN: | 1433-7851 1521-3773 |
DOI: | 10.1002/anie.200501794 |