Rourinoside and rouremin, antimalarial constituents from Rourea minor

Bioassay-directed fractionation of the CHCl 3 extract of the dried stems of Rourea minor (Gaertn.) Aubl. (Connaraceae) liana led to the isolation of two glycosides, rourinoside ( 1) and rouremin ( 2), as well as five known compounds. Compounds 1– 3 showed weak in vitro activities against Plasmodium falciparum. Their structures and stereochemistry were elucidated by spectroscopic methods and selected enzyme hydrolysis. Bioassay-directed fractionation of the antimalarial active CHCl 3 extract of the dried stems of Rourea minor (Gaertn.) Aubl. (Connaraceae) liana led to isolation of two glycosides, rourinoside ( 1) and rouremin ( 2), as well as five known compounds, 1-(26-hydroxyhexacosanoyl)-glycerol ( 3), 1- O-β- d-glucopyranosyl-(2 S,3 R,4 E-8 Z)-2- N-(2′-hydroxypalmitoyl)-octadecasphinga-4,8-dienine, 9 S,12 S,13 S-trihydroxy-10 E-octadecenoic acid, dihydrovomifoliol-9-β- d-glucopyranoside, and β-sitosterol glucoside. Compounds 1– 3 showed weak in vitro activities against Plasmodium falciparum. Their structures and stereochemistry were elucidated by spectroscopic methods and selected enzyme hydrolysis.