Effects of sodium arsenite on catalase activity, gene and protein expression in HaCaT cells

The objective of this research work is to study the effects of sodium arsenite on activity, mRNA and protein expression of catalase (CAT) in established human cell lines of keratinocytes (HaCaT). Based on the AlamarBlue assay, we found that a high level (100 μmol/l) of sodium arsenite inhibited cell...

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Veröffentlicht in:	Toxicology in vitro 2006-10, Vol.20 (7), p.1139-1144
Hauptverfasser:	Sun, Xiance, Li, Bing, Li, Xin, Wang, Yi, Xu, Yuanyuan, Jin, Yaping, Piao, Fengyuan, Sun, Guifan
Format:	Artikel
Sprache:	eng
Schlagworte:	Arsenite Arsenites - pharmacology Catalase Catalase - genetics Catalase - metabolism Cell Line Cell Shape - drug effects Cell Survival - drug effects Dose-Response Relationship, Drug Flow Cytometry - methods Gene Expression Regulation, Enzymologic - drug effects Humans Keratinocytes - cytology Keratinocytes - drug effects Keratinocytes - metabolism Oxidative stress Reactive Oxygen Species - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism Sodium Compounds - pharmacology
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Zusammenfassung:	The objective of this research work is to study the effects of sodium arsenite on activity, mRNA and protein expression of catalase (CAT) in established human cell lines of keratinocytes (HaCaT). Based on the AlamarBlue assay, we found that a high level (100 μmol/l) of sodium arsenite inhibited cell viability. The reactive oxygen species (ROS) content in cells was increased in all treated cultures dose-dependently. CAT activity, mRNA expression and protein levels were decreased by 5–20 μmol/l of sodium arsenite. It is highly suggestive that CAT gene expression and protein levels are affected by arsenic.
ISSN:	0887-2333 1879-3177
DOI:	10.1016/j.tiv.2006.02.008