A fluorogenic and chromogenic probe that detects the esterase activity of trace copper(II)

Bis(picolinoyl)fluorescein, a new fluorogenic and chromogenic probe for monitoring the esterase activity of metal ions, has been synthesized. The probe is highly selective for Cu 2+ and is applicable both to quantification of the free ion and detection of esterolytic activity of Cu bound to organic...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Bioorganic & medicinal chemistry letters 2005-11, Vol.15 (21), p.4824-4827
Hauptverfasser:	Kierat, Radoslaw M., Krämer, Roland
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Chromogenic Compounds Copper - analysis Copper - chemistry Copper - metabolism Cu(II) esterase activity Detection Enzymes and enzyme inhibitors Esterases - metabolism Fluorescence Fluorescent Dyes Fundamental and applied biological sciences. Psychology Hydrolases Hydrolysis Kinetics Ligands Medical sciences Miscellaneous Molecular Probes Pharmacology. Drug treatments Structure-Activity Relationship
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Bis(picolinoyl)fluorescein, a new fluorogenic and chromogenic probe for monitoring the esterase activity of metal ions, has been synthesized. The probe is highly selective for Cu 2+ and is applicable both to quantification of the free ion and detection of esterolytic activity of Cu bound to organic ligands or biomolecules, with a detection limit of 100 nM by fluorimetry.
ISSN:	0960-894X 1464-3405
DOI:	10.1016/j.bmcl.2005.07.042