Topical phenytoin ointment increases autograft acceptance in rats

To examine the effectiveness of topical phenytoin in preserving the skin viability and increasing acceptance rate of autograft. We conducted this study in the Central Laboratory Animal House of Jordan University of Science and Technology, Irbid, Jordan during the period from September 2004 to June 2005. Forty-two rats were divided into 2 equal groups; full thickness dorsal skin wound (4 cm2) was created in the rats. Twenty-one rats were treated with phenytoin (10% w/w ointment), the other 21 (control) were treated with standard dressing and Vaseline. An autograft was performed one week after treatment. Rats were examined for the presence of healthy granulation tissue, reduction in wound surface dimensions, and time for complete graft attachment. Phenytoin ointment had significantly increased wound bed viability and the rate of graft acceptance (p-value