Lentibacillus lacisalsi sp. nov., a moderately halophilic bacterium isolated from a saline lake in China

1 Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea 2 Environmental Biotechnology National Core Research Center, Division of Environmental Biotechnology, Gyeongsang National University, 660-701, Korea 3 Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P.R. China Correspondence Chang-Jin Kim changjin{at}kribb.re.kr An aerobic, spore-forming, moderately halophilic bacterium, strain BH260 T , was isolated from a salt lake in China. Cells of this strain were found to be motile rods with flagella. The organism grew optimally at 30–32 °C and pH 8·0 in the presence of 12–15 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate formed a clade with the type strains of the genus Lentibacillus . The levels of 16S rRNA gene sequence similarity for strain BH260 T with respect to Lentibacillus salsus KCTC 3911 T , Lentibacillus juripiscarius JCM 12147 T and Lentibacillus salicampi KCCM 41560 T were 96·0, 95·5 and 95·3 %, respectively. Chemotaxonomic data (a DNA G+C content of 44 mol%; MK-7 as the major isoprenoid quinone; A1 - meso -diaminopimelic acid as the cell-wall type; phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids; anteiso-C 15 : 0 , anteiso-C 17 : 0 and iso-C 16 : 0 as the major fatty acids) also supported assignment of the isolate to the genus Lentibacillus . On the basis of physiological and molecular properties, the isolate represents a novel species within the genus Lentibacillus , for which the name Lentibacillus lacisalsi sp. nov. is proposed. The type strain is BH260 T (=KCTC 3915 T =DSM 16462 T ). Published online ahead of print on 15 April 2005 as DOI 10.1099/ijs.0.63721-0. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strains BH260 T is AY667497 . A transmission electron micrograph of a cell of strain BH260 T is available as a supplementary figure in IJSEM Online.