Thermostable Glycosynthases and Thioglycoligases Derived from Thermotoga maritima β-Glucuronidase

By using active‐site mutants of the Thermotoga maritima β‐glucuronidase, glucuronic acid‐containing glycosides and thioglycosides have been made in high yields. These enzymes have potential use in the chemoenzymatic synthesis of a range of glycoconjugates, such as glycosaminoglycans (X=OH, NAc, H), that contain β‐linked glucuronic and galacturonic acids.