An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens

A single-step, 5-min lysis method was investigated as a rapid technique to extract genomic DNA from mycobacteria for PCR detection of M. tuberculosis directly from clinical specimens. Of 67 smear-positive clinical specimens, 64 (95.5%) were positive by PCR after this rapid extraction method.

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Bibliographische Detailangaben
Veröffentlicht in:	Journal of microbiological methods 2005-10, Vol.63 (1), p.95-98
Hauptverfasser:	Thomson, Lynn M., Traore, Hamidou, Yesilkaya, Hasan, Doig, Christine, Steingrimsdottir, Herdis, Garcia, Lucia, Forbes, Ken J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial diseases Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Detection Detergents DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Fundamental and applied biological sciences. Psychology Human bacterial diseases Humans Infectious diseases Medical sciences Microbiology Mycobacterium Mycobacterium tuberculosis Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains PCR Polymerase Chain Reaction - methods Rapid Reagent Kits, Diagnostic Sensitivity and Specificity Sputum Sputum - microbiology Tuberculosis and atypical mycobacterial infections Tuberculosis, Pulmonary - diagnosis Tuberculosis, Pulmonary - microbiology
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Beschreibung
Zusammenfassung:	A single-step, 5-min lysis method was investigated as a rapid technique to extract genomic DNA from mycobacteria for PCR detection of M. tuberculosis directly from clinical specimens. Of 67 smear-positive clinical specimens, 64 (95.5%) were positive by PCR after this rapid extraction method.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2005.03.005