Vaccine potential of influenza vectors expressing Mycobacterium tuberculosis ESAT-6 protein

We generated several attenuated recombinant influenza A vectors expressing the Mycobacterium tuberculosis early secretory antigenic target (ESAT-6) protein. The ESAT-6 protein was recently identified as one of the most promising protective antigens for cell-mediated immunity. The obtained vectors ap...

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Veröffentlicht in:Tuberculosis (Edinburgh, Scotland) Scotland), 2006-05, Vol.86 (3), p.236-246
Hauptverfasser: Stukova, M.A., Sereinig, S., Zabolotnyh, N.V., Ferko, B., Kittel, C., Romanova, J., Vinogradova, T.I., Katinger, H., Kiselev, O.I., Egorov, A.
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Sprache:eng
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Zusammenfassung:We generated several attenuated recombinant influenza A vectors expressing the Mycobacterium tuberculosis early secretory antigenic target (ESAT-6) protein. The ESAT-6 protein was recently identified as one of the most promising protective antigens for cell-mediated immunity. The obtained vectors appeared to be capable of inducing ESAT-6 specific Th1 immune response in mice after intranasal immunization. We found that double immunization with two influenza vectors of different subtypes provided a significant level of protection in mice, when applied as prophylactic vaccine, as well as substantial therapeutic effect in mice with pre-established tuberculosis infection. Moreover, we found a strong synergistic effect when vaccination with Flu/ESAT-6 vectors was combined with isoniazid treatment, resulting in a dramatic reduction of bacterial load in the lungs of infected mice.
ISSN:1472-9792
1873-281X
DOI:10.1016/j.tube.2006.01.010