Comparative evaluation of TRI-DOT Rapid HIV test with fourth-generation ELISA for the detection of human immunodeficiency virus
This study evaluated the TRI-DOT Rapid HIV test for the early detection of human immunodeficiency virus (HIV) infection in comparison with a fourth-generation ELISA (Vironostika HIV Uniform II). Of 23 609 sera, seven (0.03%) gave discordant results. Six of these were reactive only by the fourth-gene...
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| Veröffentlicht in: | Clinical microbiology and infection 2005-10, Vol.11 (10), p.850-852 |
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| container_title | Clinical microbiology and infection |
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| creator | Sudha, T. Teja, V.D. Gopal, M. Rajesh, M. Lakshmi, V. |
| description | This study evaluated the TRI-DOT Rapid HIV test for the early detection of human immunodeficiency virus (HIV) infection in comparison with a fourth-generation ELISA (Vironostika HIV Uniform II). Of 23 609 sera, seven (0.03%) gave discordant results. Six of these were reactive only by the fourth-generation assay and were p24 antigen-positive by VIDAS DUO, Western blot and qualitative RT-PCR tests. The remaining discordant serum was considered to be false-positive by the TRI-DOT assay, as it was negative by repeat ELISA and Western blot tests. The sensitivity and specificity of the TRI-DOT test were 99.48% and 99.99%, respectively, compared with the fourth-generation ELISA. |
| doi_str_mv | 10.1111/j.1469-0691.2005.01231.x |
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Of 23 609 sera, seven (0.03%) gave discordant results. Six of these were reactive only by the fourth-generation assay and were p24 antigen-positive by VIDAS DUO, Western blot and qualitative RT-PCR tests. The remaining discordant serum was considered to be false-positive by the TRI-DOT assay, as it was negative by repeat ELISA and Western blot tests. The sensitivity and specificity of the TRI-DOT test were 99.48% and 99.99%, respectively, compared with the fourth-generation ELISA.</description><identifier>ISSN: 1198-743X</identifier><identifier>EISSN: 1469-0691</identifier><identifier>DOI: 10.1111/j.1469-0691.2005.01231.x</identifier><identifier>PMID: 16153264</identifier><language>eng</language><publisher>Oxford, UK: Elsevier Ltd</publisher><subject>AIDS Serodiagnosis ; Biological and medical sciences ; Detection ; Enzyme-Linked Immunosorbent Assay ; fourth-generation ELISA ; HIV Antibodies - blood ; HIV Antigens - blood ; HIV Core Protein p24 - blood ; HIV-1 - immunology ; HIV-1 - isolation & purification ; human immunodeficiency virus ; Human viral diseases ; Immunodeficiencies ; Immunodeficiencies. Immunoglobulinopathies ; Immunoenzyme Techniques ; Immunopathology ; Infectious diseases ; Medical sciences ; p24 antigen ; Rapid assay TRI-DOT ; Sensitivity and Specificity ; Viral diseases ; Viral diseases of the lymphoid tissue and the blood. Aids</subject><ispartof>Clinical microbiology and infection, 2005-10, Vol.11 (10), p.850-852</ispartof><rights>2005 European Society of Clinical Infectious Diseases</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5371-8be5d02c00f0c93866aabeb4218660681182342d8b2eaa79fac105c0a26fbaec3</citedby><cites>FETCH-LOGICAL-c5371-8be5d02c00f0c93866aabeb4218660681182342d8b2eaa79fac105c0a26fbaec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1469-0691.2005.01231.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1469-0691.2005.01231.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17073745$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16153264$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sudha, T.</creatorcontrib><creatorcontrib>Teja, V.D.</creatorcontrib><creatorcontrib>Gopal, M.</creatorcontrib><creatorcontrib>Rajesh, M.</creatorcontrib><creatorcontrib>Lakshmi, V.</creatorcontrib><title>Comparative evaluation of TRI-DOT Rapid HIV test with fourth-generation ELISA for the detection of human immunodeficiency virus</title><title>Clinical microbiology and infection</title><addtitle>Clin Microbiol Infect</addtitle><description>This study evaluated the TRI-DOT Rapid HIV test for the early detection of human immunodeficiency virus (HIV) infection in comparison with a fourth-generation ELISA (Vironostika HIV Uniform II). Of 23 609 sera, seven (0.03%) gave discordant results. Six of these were reactive only by the fourth-generation assay and were p24 antigen-positive by VIDAS DUO, Western blot and qualitative RT-PCR tests. The remaining discordant serum was considered to be false-positive by the TRI-DOT assay, as it was negative by repeat ELISA and Western blot tests. The sensitivity and specificity of the TRI-DOT test were 99.48% and 99.99%, respectively, compared with the fourth-generation ELISA.</description><subject>AIDS Serodiagnosis</subject><subject>Biological and medical sciences</subject><subject>Detection</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>fourth-generation ELISA</subject><subject>HIV Antibodies - blood</subject><subject>HIV Antigens - blood</subject><subject>HIV Core Protein p24 - blood</subject><subject>HIV-1 - immunology</subject><subject>HIV-1 - isolation & purification</subject><subject>human immunodeficiency virus</subject><subject>Human viral diseases</subject><subject>Immunodeficiencies</subject><subject>Immunodeficiencies. Immunoglobulinopathies</subject><subject>Immunoenzyme Techniques</subject><subject>Immunopathology</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>p24 antigen</subject><subject>Rapid assay TRI-DOT</subject><subject>Sensitivity and Specificity</subject><subject>Viral diseases</subject><subject>Viral diseases of the lymphoid tissue and the blood. 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Immunoglobulinopathies</topic><topic>Immunoenzyme Techniques</topic><topic>Immunopathology</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>p24 antigen</topic><topic>Rapid assay TRI-DOT</topic><topic>Sensitivity and Specificity</topic><topic>Viral diseases</topic><topic>Viral diseases of the lymphoid tissue and the blood. Aids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sudha, T.</creatorcontrib><creatorcontrib>Teja, V.D.</creatorcontrib><creatorcontrib>Gopal, M.</creatorcontrib><creatorcontrib>Rajesh, M.</creatorcontrib><creatorcontrib>Lakshmi, V.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical microbiology and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sudha, T.</au><au>Teja, V.D.</au><au>Gopal, M.</au><au>Rajesh, M.</au><au>Lakshmi, V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative evaluation of TRI-DOT Rapid HIV test with fourth-generation ELISA for the detection of human immunodeficiency virus</atitle><jtitle>Clinical microbiology and infection</jtitle><addtitle>Clin Microbiol Infect</addtitle><date>2005-10</date><risdate>2005</risdate><volume>11</volume><issue>10</issue><spage>850</spage><epage>852</epage><pages>850-852</pages><issn>1198-743X</issn><eissn>1469-0691</eissn><abstract>This study evaluated the TRI-DOT Rapid HIV test for the early detection of human immunodeficiency virus (HIV) infection in comparison with a fourth-generation ELISA (Vironostika HIV Uniform II). Of 23 609 sera, seven (0.03%) gave discordant results. Six of these were reactive only by the fourth-generation assay and were p24 antigen-positive by VIDAS DUO, Western blot and qualitative RT-PCR tests. The remaining discordant serum was considered to be false-positive by the TRI-DOT assay, as it was negative by repeat ELISA and Western blot tests. The sensitivity and specificity of the TRI-DOT test were 99.48% and 99.99%, respectively, compared with the fourth-generation ELISA.</abstract><cop>Oxford, UK</cop><pub>Elsevier Ltd</pub><pmid>16153264</pmid><doi>10.1111/j.1469-0691.2005.01231.x</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | AIDS Serodiagnosis Biological and medical sciences Detection Enzyme-Linked Immunosorbent Assay fourth-generation ELISA HIV Antibodies - blood HIV Antigens - blood HIV Core Protein p24 - blood HIV-1 - immunology HIV-1 - isolation & purification human immunodeficiency virus Human viral diseases Immunodeficiencies Immunodeficiencies. Immunoglobulinopathies Immunoenzyme Techniques Immunopathology Infectious diseases Medical sciences p24 antigen Rapid assay TRI-DOT Sensitivity and Specificity Viral diseases Viral diseases of the lymphoid tissue and the blood. Aids |
| title | Comparative evaluation of TRI-DOT Rapid HIV test with fourth-generation ELISA for the detection of human immunodeficiency virus |
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