Molecular recognition of nucleic acids: Coralyne binds strongly to poly(A)

Molecular recognition of nucleic acids: Coralyne binds strongly to poly(A)

The small molecule coralyne was found to bind preferentially and strongly to single-stranded poly(A) with an apparent association constant ( K a) of (1.8 ± 0.3) × 10 6 M −1. Binding of coralyne to poly(A) is predominantly enthalpically driven with a stoichiometry of one coralyne per four adenine bas...

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Veröffentlicht in:FEBS letters 2005-09, Vol.579 (22), p.5035-5039
Hauptverfasser: Xing, Feifei, Song, Guangtao, Ren, Jinsong, Chaires, Jonathan B., Qu, Xiaogang
Format: Artikel
Sprache:eng
Schlagworte:
Adenosine - chemistry
Adenosine - metabolism
Animals
Berberine Alkaloids - metabolism
Binding
Circular Dichroism
Dialysis
DNA
Macromolecular Substances
Molecular Structure
Nucleic Acid Conformation
Polymers - chemistry
Polymers - metabolism
RNA
RNA, Messenger - metabolism
Secondary structure
Single-stranded poly(A)
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Zusammenfassung:The small molecule coralyne was found to bind preferentially and strongly to single-stranded poly(A) with an apparent association constant ( K a) of (1.8 ± 0.3) × 10 6 M −1. Binding of coralyne to poly(A) is predominantly enthalpically driven with a stoichiometry of one coralyne per four adenine bases. Poly(A) forms a coralyne dependent secondary structure with a melting temperature of 60 °C, for the conditions of our study.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2005.07.091