Comparative Radical Scavenging and Antidiabetic Activities of Methanolic Extract and Fractions from Achillea ligustica ALL

The yield of methanolic extract and total phenol and non polar content of flowered parts from Achillea ligustica ALL. are reported. GC-MS analysis of the non polar fraction showed that the triterpene moretenol was the major constituent (17.228%) followed by stigmast-6-en-3β-ol, veridiflorol and β-amyrin (7.524%, 5.078% 4.470%, respectively). The antioxidant activities of the methanolic extract and its fractions from A. ligustica were carried out using two different in vitro assays, 2,2-diphenyl-1-picrylhydrazyl (DPPH) test and lipid peroxidation of liposomes assay. Methanolic extract showed higher radical scavenging activity on DPPH (IC50 of 50 μg/ml). This activity is probably due to the phenolic fraction which shown an IC50 value of 22 μg/ml. A different result was obtained from the methanolic extract on the lipid peroxidation of liposomes (IC50 of 416 μg/ml). The α-amylase inhibition assay was applied to evaluate antidiabetic activity. The methanolic extract showed weak activity (28.18% at 1 mg/ml) while the n-hexane fraction showed 74.96% inhibition at 250 μg/ml.