Reverse Glycoblotting Allows Rapid-Enrichment Glycoproteomics of Biopharmaceuticals and Disease-Related Biomarkers

Identification of glycopeptides: A high‐throughput glycoproteomics approach was established by using novel reverse‐glycoblotting technology. The method involves highly selective oxidation of the terminal sialic acid residues of glycopeptides and subsequent enrichment by chemical ligation with a poly...

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Veröffentlicht in:	Angewandte Chemie (International ed.) 2007-01, Vol.46 (46), p.8808-8813
Hauptverfasser:	Kurogochi, Masaki, Amano, Maho, Fumoto, Masataka, Takimoto, Akio, Kondo, Hirosato, Nishimura, Shin-Ichiro
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-Fetoproteins - analysis Amino Acid Sequence Animals Biomarkers - analysis Biomarkers - blood Biopharmaceutics - methods Diabetes Mellitus - blood Erythropoietin - analysis Fibrinogen - analysis glycoblotting Glycopeptides - analysis glycoproteins Mice proteomics Proteomics - methods Recombinant Proteins reverse genomics sialic acids
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Beschreibung
Zusammenfassung:	Identification of glycopeptides: A high‐throughput glycoproteomics approach was established by using novel reverse‐glycoblotting technology. The method involves highly selective oxidation of the terminal sialic acid residues of glycopeptides and subsequent enrichment by chemical ligation with a polymer reagent (see schematic representation; Neu5Ac: N‐acetylneuraminic acid; Neu5Gc: N‐glycolylneuraminic acid; green: N‐acetyl‐D‐glucosamine; yellow: D‐mannose; blue: D‐galactose).
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.200702919