Medium effects on capacitation and sperm penetration through the zona pellucida in inbred BALB/c spermatozoa
Inbred BALB/c mice are one of the most difficult inbred strains to fertilize in vitro. In this study we examined the abilities of various media used for mouse in vitro fertilization (IVF) to support capacitation and sperm penetration through the zona pellucida (ZP) of inbred BALB/c spermatozoa. Medi...
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| Veröffentlicht in: | Zygote (Cambridge) 2005-05, Vol.13 (2), p.145-153 |
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| creator | Kito, Seiji Ohta, Yuki |
| description | Inbred BALB/c mice are one of the most difficult inbred strains to fertilize in vitro. In this study we examined the abilities of various media used for mouse in vitro fertilization (IVF) to support capacitation and sperm penetration through the zona pellucida (ZP) of inbred BALB/c spermatozoa. Media examined were TYH, M16, CZB, mWhitten medium, T6, modified Tyrode's solution (mTyrode's), mKSOM, MEM and TCM199. Modified human tubal fluid (mHTF) was used as a control medium. When sperm were capacitated and inseminated in the same medium, mHTF showed the best fertilization (approximately 80%) scored by male pronuclear formation ( |
| doi_str_mv | 10.1017/S0967199405003205 |
| format | Article |
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In this study we examined the abilities of various media used for mouse in vitro fertilization (IVF) to support capacitation and sperm penetration through the zona pellucida (ZP) of inbred BALB/c spermatozoa. Media examined were TYH, M16, CZB, mWhitten medium, T6, modified Tyrode's solution (mTyrode's), mKSOM, MEM and TCM199. Modified human tubal fluid (mHTF) was used as a control medium. When sperm were capacitated and inseminated in the same medium, mHTF showed the best fertilization (approximately 80%) scored by male pronuclear formation (<26%) at 5 h post-insemination (PI). When sperm were capacitated in various media and inseminated in mHTF, sperm capacitated in TYH solution (93%) but no other media (<45%) showed a significantly higher level of sperm nuclear decondensation (SND) than mHTF at 2 h PI (approximately 65%). When sperm were capacitated in mHTF and inseminated in various media, only mTyrode's (52%) was not significantly lower than mHTF (66%) in terms of SND at 2 h PI (<49%). Sperm capacitation also was examined by chlortetracycline (CTC) staining. Sperm capacitated in TYH solution showed a significantly higher percentage of capacitation (46%) than those treated in HTF (28%) and other media (<24%). These results indicate that the best approach for IVF in the BALB/c strain is capacitation in TYH and insemination in mHTF. Poor fertilization of BALB/c may result from suboptimal conditions of sperm capacitation and insemination, and overall IVF success may differ depending on strains used.</description><identifier>ISSN: 0967-1994</identifier><identifier>EISSN: 1469-8730</identifier><identifier>DOI: 10.1017/S0967199405003205</identifier><identifier>PMID: 16128410</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animals ; BALB/c inbred mice ; Capacitation ; Culture Media - pharmacology ; Fertilization in Vitro - methods ; Histological Techniques ; In vitro fertilization ; Isotonic Solutions ; Male ; Mice ; Mice, Inbred BALB C ; Oocytes - metabolism ; Sperm Capacitation - drug effects ; Spermatozoa ; Spermatozoa - drug effects ; Spermatozoa - physiology ; Zona Pellucida - physiology ; Zona penetration</subject><ispartof>Zygote (Cambridge), 2005-05, Vol.13 (2), p.145-153</ispartof><rights>2005 Cambridge University Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-cc9f5c1743f7e5bb4b3dccc527635ee18b367ae8bff2020b0d01bdef96794c13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0967199405003205/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,776,780,27903,27904,55606</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16128410$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kito, Seiji</creatorcontrib><creatorcontrib>Ohta, Yuki</creatorcontrib><title>Medium effects on capacitation and sperm penetration through the zona pellucida in inbred BALB/c spermatozoa</title><title>Zygote (Cambridge)</title><addtitle>Zygote</addtitle><description>Inbred BALB/c mice are one of the most difficult inbred strains to fertilize in vitro. In this study we examined the abilities of various media used for mouse in vitro fertilization (IVF) to support capacitation and sperm penetration through the zona pellucida (ZP) of inbred BALB/c spermatozoa. Media examined were TYH, M16, CZB, mWhitten medium, T6, modified Tyrode's solution (mTyrode's), mKSOM, MEM and TCM199. Modified human tubal fluid (mHTF) was used as a control medium. When sperm were capacitated and inseminated in the same medium, mHTF showed the best fertilization (approximately 80%) scored by male pronuclear formation (<26%) at 5 h post-insemination (PI). When sperm were capacitated in various media and inseminated in mHTF, sperm capacitated in TYH solution (93%) but no other media (<45%) showed a significantly higher level of sperm nuclear decondensation (SND) than mHTF at 2 h PI (approximately 65%). When sperm were capacitated in mHTF and inseminated in various media, only mTyrode's (52%) was not significantly lower than mHTF (66%) in terms of SND at 2 h PI (<49%). Sperm capacitation also was examined by chlortetracycline (CTC) staining. Sperm capacitated in TYH solution showed a significantly higher percentage of capacitation (46%) than those treated in HTF (28%) and other media (<24%). These results indicate that the best approach for IVF in the BALB/c strain is capacitation in TYH and insemination in mHTF. Poor fertilization of BALB/c may result from suboptimal conditions of sperm capacitation and insemination, and overall IVF success may differ depending on strains used.</description><subject>Animals</subject><subject>BALB/c inbred mice</subject><subject>Capacitation</subject><subject>Culture Media - pharmacology</subject><subject>Fertilization in Vitro - methods</subject><subject>Histological Techniques</subject><subject>In vitro fertilization</subject><subject>Isotonic Solutions</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Oocytes - metabolism</subject><subject>Sperm Capacitation - drug effects</subject><subject>Spermatozoa</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - physiology</subject><subject>Zona Pellucida - physiology</subject><subject>Zona penetration</subject><issn>0967-1994</issn><issn>1469-8730</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kV1rFDEUhoNY7Fr9Ad7I4IV30558z1x2F62Fta3Y-5BkzrSpOx8mM6D99WaZRUUpBA457_senuQQ8obCKQWqz75CrTStawESgDOQz8iKClWXlebwnKz2crnXj8nLlB4AQOtavCDHVFFWCQorsvuMTZi7AtsW_ZSKoS-8Ha0Pk51Cvti-KdKIsStG7HGKS3e6j8N8d58rFo9Db7O4280-NLYIfT4uYlOsz7frM7-k7TQ8DvYVOWrtLuHrQz0htx8_3G4-ldvri8vN-bb0klZT6X3dSk-14K1G6ZxwvPHeS6YVl4i0clxpi5VrWwYMHDRAXYNtfm0tPOUn5P0ydozD9xnTZLqQfCa0PQ5zMqqSjAsQ2fjuH-PDMMc-oxlaKy4Yk1U20cXk45BSxNaMMXQ2_jQUzH4N5r815Mzbw-DZddj8SRz-PRvKxRDShD9-6zZ-M0pzLY26-GKq-oqtN1c3Zg_BDxC2czE0d_gX6pMYvwBCVqGO</recordid><startdate>200505</startdate><enddate>200505</enddate><creator>Kito, Seiji</creator><creator>Ohta, Yuki</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>200505</creationdate><title>Medium effects on capacitation and sperm penetration through the zona pellucida in inbred BALB/c spermatozoa</title><author>Kito, Seiji ; Ohta, Yuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c518t-cc9f5c1743f7e5bb4b3dccc527635ee18b367ae8bff2020b0d01bdef96794c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>BALB/c inbred mice</topic><topic>Capacitation</topic><topic>Culture Media - pharmacology</topic><topic>Fertilization in Vitro - methods</topic><topic>Histological Techniques</topic><topic>In vitro fertilization</topic><topic>Isotonic Solutions</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Oocytes - metabolism</topic><topic>Sperm Capacitation - drug effects</topic><topic>Spermatozoa</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - physiology</topic><topic>Zona Pellucida - physiology</topic><topic>Zona penetration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kito, Seiji</creatorcontrib><creatorcontrib>Ohta, Yuki</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Zygote (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kito, Seiji</au><au>Ohta, Yuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Medium effects on capacitation and sperm penetration through the zona pellucida in inbred BALB/c spermatozoa</atitle><jtitle>Zygote (Cambridge)</jtitle><addtitle>Zygote</addtitle><date>2005-05</date><risdate>2005</risdate><volume>13</volume><issue>2</issue><spage>145</spage><epage>153</epage><pages>145-153</pages><issn>0967-1994</issn><eissn>1469-8730</eissn><abstract>Inbred BALB/c mice are one of the most difficult inbred strains to fertilize in vitro. In this study we examined the abilities of various media used for mouse in vitro fertilization (IVF) to support capacitation and sperm penetration through the zona pellucida (ZP) of inbred BALB/c spermatozoa. Media examined were TYH, M16, CZB, mWhitten medium, T6, modified Tyrode's solution (mTyrode's), mKSOM, MEM and TCM199. Modified human tubal fluid (mHTF) was used as a control medium. When sperm were capacitated and inseminated in the same medium, mHTF showed the best fertilization (approximately 80%) scored by male pronuclear formation (<26%) at 5 h post-insemination (PI). When sperm were capacitated in various media and inseminated in mHTF, sperm capacitated in TYH solution (93%) but no other media (<45%) showed a significantly higher level of sperm nuclear decondensation (SND) than mHTF at 2 h PI (approximately 65%). When sperm were capacitated in mHTF and inseminated in various media, only mTyrode's (52%) was not significantly lower than mHTF (66%) in terms of SND at 2 h PI (<49%). Sperm capacitation also was examined by chlortetracycline (CTC) staining. Sperm capacitated in TYH solution showed a significantly higher percentage of capacitation (46%) than those treated in HTF (28%) and other media (<24%). These results indicate that the best approach for IVF in the BALB/c strain is capacitation in TYH and insemination in mHTF. Poor fertilization of BALB/c may result from suboptimal conditions of sperm capacitation and insemination, and overall IVF success may differ depending on strains used.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>16128410</pmid><doi>10.1017/S0967199405003205</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0967-1994 |
| ispartof | Zygote (Cambridge), 2005-05, Vol.13 (2), p.145-153 |
| issn | 0967-1994 1469-8730 |
| language | eng |
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| source | MEDLINE; Cambridge University Press Journals Complete |
| subjects | Animals BALB/c inbred mice Capacitation Culture Media - pharmacology Fertilization in Vitro - methods Histological Techniques In vitro fertilization Isotonic Solutions Male Mice Mice, Inbred BALB C Oocytes - metabolism Sperm Capacitation - drug effects Spermatozoa Spermatozoa - drug effects Spermatozoa - physiology Zona Pellucida - physiology Zona penetration |
| title | Medium effects on capacitation and sperm penetration through the zona pellucida in inbred BALB/c spermatozoa |
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