Medium effects on capacitation and sperm penetration through the zona pellucida in inbred BALB/c spermatozoa

Inbred BALB/c mice are one of the most difficult inbred strains to fertilize in vitro. In this study we examined the abilities of various media used for mouse in vitro fertilization (IVF) to support capacitation and sperm penetration through the zona pellucida (ZP) of inbred BALB/c spermatozoa. Medi...

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Veröffentlicht in:	Zygote (Cambridge) 2005-05, Vol.13 (2), p.145-153
Hauptverfasser:	Kito, Seiji, Ohta, Yuki
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals BALB/c inbred mice Capacitation Culture Media - pharmacology Fertilization in Vitro - methods Histological Techniques In vitro fertilization Isotonic Solutions Male Mice Mice, Inbred BALB C Oocytes - metabolism Sperm Capacitation - drug effects Spermatozoa Spermatozoa - drug effects Spermatozoa - physiology Zona Pellucida - physiology Zona penetration
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Beschreibung
Zusammenfassung:	Inbred BALB/c mice are one of the most difficult inbred strains to fertilize in vitro. In this study we examined the abilities of various media used for mouse in vitro fertilization (IVF) to support capacitation and sperm penetration through the zona pellucida (ZP) of inbred BALB/c spermatozoa. Media examined were TYH, M16, CZB, mWhitten medium, T6, modified Tyrode's solution (mTyrode's), mKSOM, MEM and TCM199. Modified human tubal fluid (mHTF) was used as a control medium. When sperm were capacitated and inseminated in the same medium, mHTF showed the best fertilization (approximately 80%) scored by male pronuclear formation (
ISSN:	0967-1994 1469-8730
DOI:	10.1017/S0967199405003205