False Positive Fecal Coliform in Biosolid Samples Assayed Using A-1 Medium

Two most probable number (MPN) methods-lauryl tryptose broth with Escherichia coli broth confirmation and direct A-1 broth incubation (A-1)-were compared for the enumeration of fecal coliform in lime-treated biosolid. Fecal coliform numbers were significantly higher using the A-1 method. Analysis of...

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Veröffentlicht in:	Water environment research 2005-07, Vol.77 (4), p.423-424
Hauptverfasser:	Baker, Katherine H., Redmond, Brady, Herson, Diane S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Administrative law Applied sciences A‐1 broth Bacteria biosolid Brackish water Class A Colony Count, Microbial Comparative studies Culture Media - chemistry Effluents Enterobacteriaceae - growth & development Enterobacteriaceae - isolation & purification Environmental agencies Escherichia coli Escherichia coli - growth & development Escherichia coli - isolation & purification Exact sciences and technology False positive errors False Positive Reactions fecal coliform Fecal coliforms Feces - microbiology most probable number Other industrial wastes. Sewage sludge Pathogens Pollution Research Notes Shellfish Sludge Wastes Wastewater Water Microbiology Water treatment
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Zusammenfassung:	Two most probable number (MPN) methods-lauryl tryptose broth with Escherichia coli broth confirmation and direct A-1 broth incubation (A-1)-were compared for the enumeration of fecal coliform in lime-treated biosolid. Fecal coliform numbers were significantly higher using the A-1 method. Analysis of positive A-1 tubes, however, indicated that a high percentage of these were false positives. Therefore, the use of A-1 broth for 40 CFR Part 503 Pathogen Reduction (CFR, 1993) compliance testing is not recommended.
ISSN:	1061-4303 1554-7531
DOI:	10.1002/j.1554-7531.2005.tb00302.x