Sequence specific interstrand photocrosslinking for effective SNP typing

We describe a simple and inexpensive SNP typing method by using sequence specific interstrand photocrosslinking via p-carbamoylvinyl phenol nucleosides. Interstrand photocrosslinking showed a high degree of single nucleotide specificity as high as 10(3)-fold and more, and can be used in the diagnost...

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Veröffentlicht in:	Organic & biomolecular chemistry 2007-01, Vol.5 (16), p.2583-2586
Hauptverfasser:	Ami, Takehiro, Ito, Katsuya, Yoshimura, Yoshinaga, Fujimoto, Kenzo
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Cross-Linking Reagents - chemistry Cross-Linking Reagents - radiation effects DNA - chemistry DNA - radiation effects Nucleic Acid Conformation Nucleosides - chemistry Nucleosides - radiation effects Oligodeoxyribonucleotides - chemistry Oligonucleotide Array Sequence Analysis - methods Phenols - chemistry Phenols - radiation effects Photochemistry Polymorphism, Single Nucleotide - radiation effects Sequence Analysis, DNA - methods Substrate Specificity Ultraviolet Rays
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Zusammenfassung:	We describe a simple and inexpensive SNP typing method by using sequence specific interstrand photocrosslinking via p-carbamoylvinyl phenol nucleosides. Interstrand photocrosslinking showed a high degree of single nucleotide specificity as high as 10(3)-fold and more, and can be used in the diagnostic detection of DNA sequences.
ISSN:	1477-0520 1477-0539
DOI:	10.1039/b708264a