Development of a sensitive enzyme immunoassay for anti-Müllerian hormone and the evaluation of potential clinical applications in males and females

Summary Background and objective Recent studies have found anti‐Müllerian hormone (AMH) to be a potentially important marker for the assessment of ovarian reserve and prediction of the success of in vitro fertilization (IVF) treatment. The objectives of this study were to develop a sensitive and specific assay for AMH and to evaluate the potential application of the assay. This assay will be then available to our collaborators in the UK and overseas. Design Samples obtained as part of another prospective cross‐sectional study from infertility patients and another prospective longitudinal study from pregnant women were used in this study to measure AMH using a new double‐antibody enzyme‐linked immunosorbent assay (ELISA). Patients and measurements AMH levels were evaluated in (i) serum and seminal fluid from males (normal and male factor infertility males), (ii) serum and follicular fluid from females (normal and female with unexplained infertility) and (iii) serum, amniotic fluid (AF) and coelomic fluid (CF) from pregnant women. AMH levels in the samples were measured by a newly developed ELISA. Result The assay had a detection limit of