A new method of culturing Leydig cells and prospects of their practical use in andrology

A new method of culturing Leydig cells and prospects of their practical use in andrology

We present a new method of obtaining a xenoculture enriched with Leydig cells from neonatal porcine testes providing 97-99% viability of cells in the culture. Genital extra-testicular transplantation of the obtained xenoculture was applied, which minimized traumaticity of surgery and the number of p...

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Veröffentlicht in:Bulletin of experimental biology and medicine 2007-01, Vol.143 (1), p.155-159
Hauptverfasser: Artyukhin, A A, Zaraiskii, E I
Format: Artikel
Sprache:eng
Schlagworte:
Adult
Animals
Animals, Newborn
Cell Culture Techniques
Cell Survival
Cell Transplantation
Cells, Cultured
Humans
Infertility - immunology
Infertility - therapy
Leydig Cells - cytology
Male
Ozone - therapeutic use
Spermatic Cord - cytology
Swine
Transplantation, Heterologous
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Zusammenfassung:We present a new method of obtaining a xenoculture enriched with Leydig cells from neonatal porcine testes providing 97-99% viability of cells in the culture. Genital extra-testicular transplantation of the obtained xenoculture was applied, which minimized traumaticity of surgery and the number of postoperative complications and had undoubted advantages over the subcapsular cell culture depot.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-007-0038-6