Adenoviral infection of survivin antisense sensitizes prostate cancer cells to etoposide in vivo
BACKGROUND We aimed to investigate whether use of a survivin antisense fragment carried by an adenovirus vector (Ad.survivin‐AS) could enhance the therapeutic efficacy of chemotherapy for androgen‐independent prostate cancer. METHODS We used Ad.survivin‐AS to promote apoptosis through inhibition of...
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| Veröffentlicht in: | The Prostate 2005-09, Vol.65 (1), p.10-19 |
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| creator | Hayashi, Norihiro Asano, Koji Suzuki, Hideaki Yamamoto, Tetsuhisa Tanigawa, Nobuhiko Egawa, Shin Manome, Yoshinobu |
| description | BACKGROUND
We aimed to investigate whether use of a survivin antisense fragment carried by an adenovirus vector (Ad.survivin‐AS) could enhance the therapeutic efficacy of chemotherapy for androgen‐independent prostate cancer.
METHODS
We used Ad.survivin‐AS to promote apoptosis through inhibition of survivin expression. Recombinant adenoviruses alone or in combination with chemotherapeutic agents were tested for anti‐cancer activity both in vitro and in vivo.
RESULTS
Infection with Ad.survivin‐AS strongly inhibited survivin expression in a dose‐ and time‐dependent manner, resulting in significant antitumor activity in vitro and in vivo. Downregulation of survivin expression potentiated induction of apoptosis by the chemotherapeutic agents docetaxel and etoposide in DU145 cells. In particular, the combination of etoposide and Ad.survivin‐AS demonstrated dramatic growth inhibition with no tumor regrowth being observed during the experimental period.
CONCLUSIONS
The prominent synergy of this combination may provide a basis for clinical application of Ad.survivin‐AS as a chemosensitizer of etoposide. © 2005 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/pros.20232 |
| format | Article |
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We aimed to investigate whether use of a survivin antisense fragment carried by an adenovirus vector (Ad.survivin‐AS) could enhance the therapeutic efficacy of chemotherapy for androgen‐independent prostate cancer.
METHODS
We used Ad.survivin‐AS to promote apoptosis through inhibition of survivin expression. Recombinant adenoviruses alone or in combination with chemotherapeutic agents were tested for anti‐cancer activity both in vitro and in vivo.
RESULTS
Infection with Ad.survivin‐AS strongly inhibited survivin expression in a dose‐ and time‐dependent manner, resulting in significant antitumor activity in vitro and in vivo. Downregulation of survivin expression potentiated induction of apoptosis by the chemotherapeutic agents docetaxel and etoposide in DU145 cells. In particular, the combination of etoposide and Ad.survivin‐AS demonstrated dramatic growth inhibition with no tumor regrowth being observed during the experimental period.
CONCLUSIONS
The prominent synergy of this combination may provide a basis for clinical application of Ad.survivin‐AS as a chemosensitizer of etoposide. © 2005 Wiley‐Liss, Inc.</description><identifier>ISSN: 0270-4137</identifier><identifier>EISSN: 1097-0045</identifier><identifier>DOI: 10.1002/pros.20232</identifier><identifier>PMID: 15799033</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adenoviridae - genetics ; adenovirus vector ; Animals ; antisense ; Antisense Elements (Genetics) - pharmacology ; Apoptosis ; Cell Line, Tumor ; Cell Proliferation ; chemotherapy ; Etoposide - pharmacology ; Humans ; Inhibitor of Apoptosis Proteins ; Male ; Mice ; Mice, Inbred BALB C ; Microtubule-Associated Proteins - antagonists & inhibitors ; Microtubule-Associated Proteins - genetics ; Neoplasm Proteins - antagonists & inhibitors ; Neoplasm Proteins - genetics ; Neoplasm Transplantation ; prostate cancer ; Prostatic Neoplasms - drug therapy ; Prostatic Neoplasms - pathology ; survivin ; Taxoids - pharmacology ; Transplantation, Heterologous</subject><ispartof>The Prostate, 2005-09, Vol.65 (1), p.10-19</ispartof><rights>Copyright © 2005 Wiley‐Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4312-2d1acfb9bcb026e8a3cf5aaf306bea11ac702623b8c24313c5da0b30a4fb77c63</citedby><cites>FETCH-LOGICAL-c4312-2d1acfb9bcb026e8a3cf5aaf306bea11ac702623b8c24313c5da0b30a4fb77c63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpros.20232$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpros.20232$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15799033$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hayashi, Norihiro</creatorcontrib><creatorcontrib>Asano, Koji</creatorcontrib><creatorcontrib>Suzuki, Hideaki</creatorcontrib><creatorcontrib>Yamamoto, Tetsuhisa</creatorcontrib><creatorcontrib>Tanigawa, Nobuhiko</creatorcontrib><creatorcontrib>Egawa, Shin</creatorcontrib><creatorcontrib>Manome, Yoshinobu</creatorcontrib><title>Adenoviral infection of survivin antisense sensitizes prostate cancer cells to etoposide in vivo</title><title>The Prostate</title><addtitle>Prostate</addtitle><description>BACKGROUND
We aimed to investigate whether use of a survivin antisense fragment carried by an adenovirus vector (Ad.survivin‐AS) could enhance the therapeutic efficacy of chemotherapy for androgen‐independent prostate cancer.
METHODS
We used Ad.survivin‐AS to promote apoptosis through inhibition of survivin expression. Recombinant adenoviruses alone or in combination with chemotherapeutic agents were tested for anti‐cancer activity both in vitro and in vivo.
RESULTS
Infection with Ad.survivin‐AS strongly inhibited survivin expression in a dose‐ and time‐dependent manner, resulting in significant antitumor activity in vitro and in vivo. Downregulation of survivin expression potentiated induction of apoptosis by the chemotherapeutic agents docetaxel and etoposide in DU145 cells. In particular, the combination of etoposide and Ad.survivin‐AS demonstrated dramatic growth inhibition with no tumor regrowth being observed during the experimental period.
CONCLUSIONS
The prominent synergy of this combination may provide a basis for clinical application of Ad.survivin‐AS as a chemosensitizer of etoposide. © 2005 Wiley‐Liss, Inc.</description><subject>Adenoviridae - genetics</subject><subject>adenovirus vector</subject><subject>Animals</subject><subject>antisense</subject><subject>Antisense Elements (Genetics) - pharmacology</subject><subject>Apoptosis</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>chemotherapy</subject><subject>Etoposide - pharmacology</subject><subject>Humans</subject><subject>Inhibitor of Apoptosis Proteins</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microtubule-Associated Proteins - antagonists & inhibitors</subject><subject>Microtubule-Associated Proteins - genetics</subject><subject>Neoplasm Proteins - antagonists & inhibitors</subject><subject>Neoplasm Proteins - genetics</subject><subject>Neoplasm Transplantation</subject><subject>prostate cancer</subject><subject>Prostatic Neoplasms - drug therapy</subject><subject>Prostatic Neoplasms - pathology</subject><subject>survivin</subject><subject>Taxoids - pharmacology</subject><subject>Transplantation, Heterologous</subject><issn>0270-4137</issn><issn>1097-0045</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kLtOwzAUhi0EgnJZeADkiQEp5dhu4mZECAoSAsSljMZxTiRDGpfYLZSnx6EFNhZbsr__OxdC9hn0GQA_nrbO9zlwwddIj0EuE4BBuk56wCUkAybkFtn2_gUg4sA3yRZLZZ6DED3yfFJi4-a21TW1TYUmWNdQV1E_a-d2bhuqm2A9Nh5pd9pgP9HTrmTQAanRjcGWGqxrT4OjGNzUeVtitNEocLtko9K1x73VvUMez88eTi-Sq5vR5enJVWIGgvGEl0ybqsgLUwDPcKiFqVKtKwFZgZrFTxnfuSiGhseAMGmpoRCgB1UhpcnEDjlcemNrbzP0QU2s79rSDbqZV9kwBRgCRPBoCZo4g2-xUtPWTnS7UAxUt0_VDae-9xnhg5V1Vkyw_ENXC4wAWwLvtsbFPyp1e3dz_yNNlhnrA378ZnT7qjIpZKqerkcqH4_z8fVDqu7FFylLklE</recordid><startdate>20050915</startdate><enddate>20050915</enddate><creator>Hayashi, Norihiro</creator><creator>Asano, Koji</creator><creator>Suzuki, Hideaki</creator><creator>Yamamoto, Tetsuhisa</creator><creator>Tanigawa, Nobuhiko</creator><creator>Egawa, Shin</creator><creator>Manome, Yoshinobu</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050915</creationdate><title>Adenoviral infection of survivin antisense sensitizes prostate cancer cells to etoposide in vivo</title><author>Hayashi, Norihiro ; Asano, Koji ; Suzuki, Hideaki ; Yamamoto, Tetsuhisa ; Tanigawa, Nobuhiko ; Egawa, Shin ; Manome, Yoshinobu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4312-2d1acfb9bcb026e8a3cf5aaf306bea11ac702623b8c24313c5da0b30a4fb77c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adenoviridae - genetics</topic><topic>adenovirus vector</topic><topic>Animals</topic><topic>antisense</topic><topic>Antisense Elements (Genetics) - pharmacology</topic><topic>Apoptosis</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>chemotherapy</topic><topic>Etoposide - pharmacology</topic><topic>Humans</topic><topic>Inhibitor of Apoptosis Proteins</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microtubule-Associated Proteins - antagonists & inhibitors</topic><topic>Microtubule-Associated Proteins - genetics</topic><topic>Neoplasm Proteins - antagonists & inhibitors</topic><topic>Neoplasm Proteins - genetics</topic><topic>Neoplasm Transplantation</topic><topic>prostate cancer</topic><topic>Prostatic Neoplasms - drug therapy</topic><topic>Prostatic Neoplasms - pathology</topic><topic>survivin</topic><topic>Taxoids - pharmacology</topic><topic>Transplantation, Heterologous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hayashi, Norihiro</creatorcontrib><creatorcontrib>Asano, Koji</creatorcontrib><creatorcontrib>Suzuki, Hideaki</creatorcontrib><creatorcontrib>Yamamoto, Tetsuhisa</creatorcontrib><creatorcontrib>Tanigawa, Nobuhiko</creatorcontrib><creatorcontrib>Egawa, Shin</creatorcontrib><creatorcontrib>Manome, Yoshinobu</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Prostate</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hayashi, Norihiro</au><au>Asano, Koji</au><au>Suzuki, Hideaki</au><au>Yamamoto, Tetsuhisa</au><au>Tanigawa, Nobuhiko</au><au>Egawa, Shin</au><au>Manome, Yoshinobu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adenoviral infection of survivin antisense sensitizes prostate cancer cells to etoposide in vivo</atitle><jtitle>The Prostate</jtitle><addtitle>Prostate</addtitle><date>2005-09-15</date><risdate>2005</risdate><volume>65</volume><issue>1</issue><spage>10</spage><epage>19</epage><pages>10-19</pages><issn>0270-4137</issn><eissn>1097-0045</eissn><abstract>BACKGROUND
We aimed to investigate whether use of a survivin antisense fragment carried by an adenovirus vector (Ad.survivin‐AS) could enhance the therapeutic efficacy of chemotherapy for androgen‐independent prostate cancer.
METHODS
We used Ad.survivin‐AS to promote apoptosis through inhibition of survivin expression. Recombinant adenoviruses alone or in combination with chemotherapeutic agents were tested for anti‐cancer activity both in vitro and in vivo.
RESULTS
Infection with Ad.survivin‐AS strongly inhibited survivin expression in a dose‐ and time‐dependent manner, resulting in significant antitumor activity in vitro and in vivo. Downregulation of survivin expression potentiated induction of apoptosis by the chemotherapeutic agents docetaxel and etoposide in DU145 cells. In particular, the combination of etoposide and Ad.survivin‐AS demonstrated dramatic growth inhibition with no tumor regrowth being observed during the experimental period.
CONCLUSIONS
The prominent synergy of this combination may provide a basis for clinical application of Ad.survivin‐AS as a chemosensitizer of etoposide. © 2005 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>15799033</pmid><doi>10.1002/pros.20232</doi><tpages>10</tpages></addata></record> |
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| subjects | Adenoviridae - genetics adenovirus vector Animals antisense Antisense Elements (Genetics) - pharmacology Apoptosis Cell Line, Tumor Cell Proliferation chemotherapy Etoposide - pharmacology Humans Inhibitor of Apoptosis Proteins Male Mice Mice, Inbred BALB C Microtubule-Associated Proteins - antagonists & inhibitors Microtubule-Associated Proteins - genetics Neoplasm Proteins - antagonists & inhibitors Neoplasm Proteins - genetics Neoplasm Transplantation prostate cancer Prostatic Neoplasms - drug therapy Prostatic Neoplasms - pathology survivin Taxoids - pharmacology Transplantation, Heterologous |
| title | Adenoviral infection of survivin antisense sensitizes prostate cancer cells to etoposide in vivo |
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