Analysis of the distribution and diversity in recent Hawaiian volcanic deposits of a putative carbon monoxide dehydrogenase large subunit gene

Analysis of the distribution and diversity in recent Hawaiian volcanic deposits of a putative carbon monoxide dehydrogenase large subunit gene

Summary A putative carbon monoxide dehydrogenase large subunit gene (BMS putative coxL) was amplified from genomic DNA extracts of four recent (42–300 year old) Hawaiian volcanic deposits by polymerase chain reaction (PCR). Sequence databases derived from clone libraries constructed using PCR produc...

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Veröffentlicht in:Environmental microbiology 2005-09, Vol.7 (9), p.1405-1412
Hauptverfasser: Dunfield, Kari E., King, Gary M.
Format: Artikel
Sprache:eng
Schlagworte:
Aldehyde Oxidoreductases - genetics
Bacterial Proteins - genetics
Base Sequence
DNA, Bacterial - analysis
Genetic Variation
Geologic Sediments - microbiology
Hawaii
Molecular Sequence Data
Multienzyme Complexes - genetics
Phylogeny
Polymerase Chain Reaction
Protein Subunits - genetics
Proteobacteria - classification
Proteobacteria - enzymology
Proteobacteria - genetics
Time Factors
Volcanic Eruptions
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Zusammenfassung:Summary A putative carbon monoxide dehydrogenase large subunit gene (BMS putative coxL) was amplified from genomic DNA extracts of four recent (42–300 year old) Hawaiian volcanic deposits by polymerase chain reaction (PCR). Sequence databases derived from clone libraries constructed using PCR products were analysed phylogenetically and statistically. These analyses indicated that each of the deposits supported distinct BMS putative coxL gene assemblages. Statistical analyses also showed that the youngest deposit (42 years old) contained the least diverse sequences (P 
ISSN:1462-2912
1462-2920
DOI:10.1111/j.1462-2920.2005.00827.x