Comparison of Plaque- and Flow Cytometry-Based Methods for Measuring Dengue Virus Neutralization

As dengue vaccines enter clinical trials, there is a need for rapid and quantitative assays to measure neutralization. We have developed flow-based neutralization assays which generated results similar to those generated by the established, plaque reduction neutralization test. The flow assays are a...

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Veröffentlicht in:	Journal of Clinical Microbiology 2007-11, Vol.45 (11), p.3777-3780
Hauptverfasser:	Kraus, Annette A, Messer, William, Haymore, Laura B, de Silva, Aravinda M
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Dengue Vaccines - immunology Dengue virus Dengue Virus - immunology Flow Cytometry - methods Fundamental and applied biological sciences. Psychology Humans Immune Sera - immunology Microbiology Miscellaneous Neutralization Tests - methods Viral Plaque Assay Virology
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Zusammenfassung:	As dengue vaccines enter clinical trials, there is a need for rapid and quantitative assays to measure neutralization. We have developed flow-based neutralization assays which generated results similar to those generated by the established, plaque reduction neutralization test. The flow assays are an improvement, as they use human cells and allow for high-throughput screening.
ISSN:	0095-1137 1098-660X 1098-5530
DOI:	10.1128/JCM.00827-07