Rapid determination of viral RNA sequences in mosquitoes collected in the field

A method for rapid determination of viral RNA sequences (RDV) was applied to homogenates of Aedes aegypti collected in Thailand in an area in which dengue fever (dengue hemorrhagic fever) is endemic, using the mosquito cell line C6/36. Nucleic acid sequences of dengue virus type 4 and cell fusing ag...

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Veröffentlicht in:	Journal of virological methods 2007-12, Vol.146 (1), p.372-374
Hauptverfasser:	Kihara, Yuki, Satho, Tomomitsu, Eshita, Yuki, Sakai, Kouji, Kotaki, Akira, Takasaki, Tomohiko, Rongsriyam, Yupha, Komalamisra, Narumon, Srisawat, Raweewan, Lapcharoen, Parichat, Sumroiphon, Suchada, Iwanaga, Shiroh, Ushijima, Hiroshi, Endoh, Daiji, Miyata, Takeshi, Sakata, Akira, Kashige, Nobuhiro, Miake, Fumio, Fukushi, Shuetsu, Saijo, Masayuki, Kurane, Ichiro, Morikawa, Shigeru, Mizutani, Tetsuya
Format:	Artikel
Sprache:	eng
Schlagworte:	Aedes - virology Aedes aegypti Animals Biological and medical sciences Cell fusing agent virus Cell Line Dengue Virus - genetics Dengue Virus - isolation & purification Dengue virus type 4 Female Fundamental and applied biological sciences. Psychology Microbiology Rapid determination Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Viral - analysis Severe Dengue - virology Techniques used in virology Thailand Virology
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Beschreibung
Zusammenfassung:	A method for rapid determination of viral RNA sequences (RDV) was applied to homogenates of Aedes aegypti collected in Thailand in an area in which dengue fever (dengue hemorrhagic fever) is endemic, using the mosquito cell line C6/36. Nucleic acid sequences of dengue virus type 4 and cell fusing agent virus were detected. This RDV method has the potential to become a standard method for detection of both known and newly emerging, unknown mosquito-borne viruses.
ISSN:	0166-0934 1879-0984
DOI:	10.1016/j.jviromet.2007.07.008