Effects of PKC ζ on early genome transcription activation in mouse 1-cell stage fertilized eggs

Effects of PKC ζ on the activation of embryonic transcription in 1‐cell stage fertilized mouse eggs were explored. The effects of PKC antagonist calphostin C and PKC ζ specific inhibitor on the activation of embryonic early transcription were observed by Western blotting and cell immunofluorescence....

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Veröffentlicht in:	Cell biochemistry and function 2007-11, Vol.25 (6), p.619-624
Hauptverfasser:	Yu, Bing-zhi, Fu, Wei, Su, Wen-hui, Yu, Da-hai, Zhang, Zhe, Feng, Chen
Format:	Artikel
Sprache:	eng
Schlagworte:	1-cell stage fertilized eggs Animals Blotting, Western calphostin C Enzyme Inhibitors - pharmacology Female G1 Phase G2 Phase Gene Expression Regulation, Developmental - drug effects Genome Male Mice Naphthalenes - pharmacology Phosphorylation Pregnancy Protein Kinase C - antagonists & inhibitors Protein Kinase C - metabolism protein kinase C ζ Threonine - metabolism transcription Transcriptional Activation - drug effects Transcriptional Activation - genetics Zygote - cytology Zygote - drug effects Zygote - metabolism
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Zusammenfassung:	Effects of PKC ζ on the activation of embryonic transcription in 1‐cell stage fertilized mouse eggs were explored. The effects of PKC antagonist calphostin C and PKC ζ specific inhibitor on the activation of embryonic early transcription were observed by Western blotting and cell immunofluorescence. PKC activity increased gradually from G1 phase to late G2 phase in mouse 1‐cell stage fertilized eggs, and reached a maximum in G2 stage. Calphostin C inhibited PKC activity by about 47% in 1‐cell stage fertilized eggs. Calphostin C inhibited early transcription in 1‐cell stage fertilized eggs (p
ISSN:	0263-6484 1099-0844
DOI:	10.1002/cbf.1357