Bead-Based Cellular Analysis, Sorting and Multiplexing

Bead-Based Cellular Analysis, Sorting and Multiplexing

Functionalized cross‐linked polystyrene microspheres were synthesized, fluorescently labelled and delivered into cells to function as cellular tags and probes (see figure). This allowed the effective delivery of foreign materials into intact mammalian cells, without the need for delicate procedures...

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Veröffentlicht in:Chembiochem : a European journal of chemical biology 2005-08, Vol.6 (8), p.1341-1345
Hauptverfasser: Sanchez-Martin, Rosario M, Muzerelle, Mathilde, Chitkul, Nutcha, How, Siew Eng, Mittoo, Stifun, Bradley, Mark
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biopolymers
Cell Line
Cell Line, Tumor
Cell Separation - methods
drug delivery
Flow Cytometry - methods
Fluorescent Dyes - chemistry
fluorescent probes
Green Fluorescent Proteins - metabolism
Humans
in vitro screening
Microspheres
multifunctionalized microspheres
Particle Size
Polystyrenes - chemistry
Rhodamines - chemistry
Xanthenes - chemistry
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Beschreibung
Zusammenfassung:Functionalized cross‐linked polystyrene microspheres were synthesized, fluorescently labelled and delivered into cells to function as cellular tags and probes (see figure). This allowed the effective delivery of foreign materials into intact mammalian cells, without the need for delicate procedures such as micro‐injection, and did not disrupt cell physiology.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.200500059