Fluorescence correlation spectroscopy in living cells

Fluorescence correlation spectroscopy in living cells

Fluorescence correlation spectroscopy (FCS) is an ideal analytical tool for studying concentrations, propagation, interactions and internal dynamics of molecules at nanomolar concentrations in living cells. FCS analyzes minute fluorescence-intensity fluctuations about the equilibrium of a small ense...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Nature methods 2007-11, Vol.4 (11), p.963-973
Hauptverfasser: Schwille, Petra, Kim, Sally A, Heinze, Katrin G
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Bioinformatics
Biological Microscopy
Biological Techniques
Biological Transport
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Cellular biology
Cellular control mechanisms
Cytophotometry - instrumentation
Cytophotometry - methods
Eukaryotic Cells - metabolism
Fluorescence
Fluorescence spectroscopy
Fluorescent Dyes - chemistry
Humans
Kinetics
Life Sciences
Models, Biological
Molecular biology
Molecular dynamics
Proteins - chemistry
Proteins - metabolism
Proteomics
protocol
Research methodology
Spectrometry, Fluorescence - instrumentation
Spectrometry, Fluorescence - methods
Spectroscopy
Spectrum analysis
Staining and Labeling
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Fluorescence correlation spectroscopy (FCS) is an ideal analytical tool for studying concentrations, propagation, interactions and internal dynamics of molecules at nanomolar concentrations in living cells. FCS analyzes minute fluorescence-intensity fluctuations about the equilibrium of a small ensemble (
ISSN:1548-7091
1548-7105
DOI:10.1038/nmeth1104